The infection of mammalian cells by viruses and innate immune responses to infection are
spatiotemporally organized processes. Cytosolic RNA sensors trigger nuclear translocation …

Over the last several years a wealth of transformative human–virus interaction discoveries
have been produced using loss-of-function functional genomics. These insights have greatly …

It is highly desirable to identify gene's function in a high-throughput fashion, and the
CRISPR/Cas9 system has been harnessed to meet such a need. Here, we describe a …

Pooled optical screens have enabled the study of cellular interactions, morphology, or
dynamics at massive scale, but have not yet leveraged the power of highly-plexed single …

Studies of the host response to virus infection typically focus on protein-coding genes.
However, non-protein-coding RNAs (ncRNAs) are transcribed in mammalian cells, and the …

Several groups have used genome-wide libraries of lentiviruses encoding small guide
RNAs (sgRNAs) for genetic screens. In most cases, sgRNA expression cassettes are …

Host: pathogen interactions dictate the outcome of infection, yet the limitations of current
approaches leave large regions of this interface unexplored. Here, we develop a novel …

Broad-spectrum antiviral drugs targeting host processes could potentially treat a wide range
of viruses while reducing the likelihood of emergent resistance. Despite great promise as …

Herpesvirus infection initiates a range of perturbations in the host cell, which remain poorly
understood at the level of individual cells. Here, we quantify the transcriptome of single …

Understanding how viral and host factors interact and how perturbations impact infection is
the basis for designing antiviral interventions. Here we define the functional contribution of …