[HTML][HTML] A sensitive approach to map genome-wide 5-hydroxymethylcytosine and 5-formylcytosine at single-base resolution
Summary Mapping genome-wide 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine
(5fC) at single-base resolution is important to understand their biological functions. We …
(5fC) at single-base resolution is important to understand their biological functions. We …
[HTML][HTML] Base-resolution analysis of 5-hydroxymethylcytosine in the mammalian genome
Summary The study of 5-hydroxylmethylcytosines (5hmC) has been hampered by the lack of
a method to map it at single-base resolution on a genome-wide scale. Affinity purification …
a method to map it at single-base resolution on a genome-wide scale. Affinity purification …
Quantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution
5-Methylcytosine can be converted to 5-hydroxymethylcytosine (5hmC) in mammalian DNA
by the ten-eleven translocation (TET) enzymes. We introduce oxidative bisulfite sequencing …
by the ten-eleven translocation (TET) enzymes. We introduce oxidative bisulfite sequencing …
Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution
Recently, the cytosine modifications 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine
(5fC) were found to exist in the genomic deoxyribonucleic acid (DNA) of a wide range of …
(5fC) were found to exist in the genomic deoxyribonucleic acid (DNA) of a wide range of …
[HTML][HTML] Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing
P Gibas, M Narmontė, Z Staševskij, J Gordevičius… - PLoS …, 2020 - journals.plos.org
5-hydroxymethylcytosine (5hmC) is the most prevalent intermediate on the oxidative DNA
demethylation pathway and is implicated in regulation of embryogenesis, neurological …
demethylation pathway and is implicated in regulation of embryogenesis, neurological …
[HTML][HTML] RRHP: a tag-based approach for 5-hydroxymethylcytosine mapping at single-site resolution
A Petterson, TH Chung, D Tan, X Sun, XY Jia - Genome biology, 2014 - Springer
Current methods for genomic mapping of 5-hydroxymethylcytosine (5hmC) have been
limited by either costly sequencing depth, high DNA input, or lack of single-base resolution …
limited by either costly sequencing depth, high DNA input, or lack of single-base resolution …
Enzymatic approaches and bisulfite sequencing cannot distinguish between 5-methylcytosine and 5-hydroxymethylcytosine in DNA
DNA cytosine methylation (5mC) is highly abundant in mammalian cells and is associated
with transcriptional repression. Recently, hydroxymethylcytosine (hmC) has been detected …
with transcriptional repression. Recently, hydroxymethylcytosine (hmC) has been detected …
Jump-seq: genome-wide capture and amplification of 5-hydroxymethylcytosine sites
5-Hydroxymethylcytosine (5hmC) arises from the oxidation of 5-methylcytosine (5mC) by
Fe2+ and 2-oxoglutarate-dependent 10–11 translocation (TET) family proteins. Substantial …
Fe2+ and 2-oxoglutarate-dependent 10–11 translocation (TET) family proteins. Substantial …
Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine
A complete understanding of the potential function of 5-hydroxymethylcytosine (5-hmC), a
DNA cytosine modification in mammalian cells, requires an accurate single-base resolution …
DNA cytosine modification in mammalian cells, requires an accurate single-base resolution …
[HTML][HTML] Tissue-specific 5-hydroxymethylcytosine landscape of the human genome
Hydroxymethylcytosine (5hmC) is an important epigenetic mark that regulates gene
expression. Charting the landscape of 5hmC in human tissues is fundamental to …
expression. Charting the landscape of 5hmC in human tissues is fundamental to …