Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may
cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is …

Genome editing through the delivery of CRISPR/Cas9-ribonucleoprotein (Cas9-RNP)
reduces unwanted gene targeting and avoids integrational mutagenesis that can occur …

CRISPR-Cas9 has emerged as a powerful technology that relies on Cas9/sgRNA
ribonucleoprotein complexes (RNPs) to target and edit DNA. However, many therapeutic …

Abstract Streptococcus pyogenes (Spy) Cas9 has potential as a component of gene
therapeutics for incurable diseases. One of its limitations is its large size, which impedes its …

Implementation of therapeutic in vivo gene editing using CRISPR/Cas relies on potent
delivery of gene editing tools. Administration of ribonucleoprotein (RNP) complexes …

CRISPR-Cas9 is a versatile genome-editing technology that is a promising gene therapy
tactic. However, the delivery of CRISPR-Cas9 is still a major obstacle to its broader clinical …

The CRISPR-Cas9 system is a powerful gene-editing tool with wide-ranging applications,
but the safe and efficient intracellular delivery of CRISPR components remains a challenge …

Recent innovations in genome editing have enabled the precise manipulation of the genetic
information of mammalians, and benefitted the development of next‐generation gene …

CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years,
however, the translation of this biotechnology into therapy has been hindered by efficient …

The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-
associated) nuclease system represents an efficient tool for genome editing and gene …