Assessing genome assembly quality using the LTR Assembly Index (LAI)
Assembling a plant genome is challenging due to the abundance of repetitive sequences,
yet no standard is available to evaluate the assembly of repeat space. LTR retrotransposons …
yet no standard is available to evaluate the assembly of repeat space. LTR retrotransposons …
Versatile genome assembly evaluation with QUAST-LG
Motivation The emergence of high-throughput sequencing technologies revolutionized
genomics in early 2000s. The next revolution came with the era of long-read sequencing …
genomics in early 2000s. The next revolution came with the era of long-read sequencing …
LTR_FINDER_parallel: parallelization of LTR_FINDER enabling rapid identification of long terminal repeat retrotransposons
Annotation of plant genomes is still a challenging task due to the abundance of repetitive
sequences, especially long terminal repeat (LTR) retrotransposons. LTR_FINDER is a …
sequences, especially long terminal repeat (LTR) retrotransposons. LTR_FINDER is a …
gEVAL—a web-based browser for evaluating genome assemblies
Motivation: For most research approaches, genome analyses are dependent on the
existence of a high quality genome reference assembly. However, the local accuracy of an …
existence of a high quality genome reference assembly. However, the local accuracy of an …
RNA-seq assembly–are we there yet?
Transcriptomic sequence resources represent invaluable assets for research, in particular
for non-model species without a sequenced genome. To date, the Next Generation …
for non-model species without a sequenced genome. To date, the Next Generation …
Tools and strategies for long-read sequencing and de novo assembly of plant genomes
The commercial release of third-generation sequencing technologies (TGSTs), giving long
and ultra-long sequencing reads, has stimulated the development of new tools for …
and ultra-long sequencing reads, has stimulated the development of new tools for …
LR_Gapcloser: a tiling path-based gap closer that uses long reads to complete genome assembly
GC Xu, TJ Xu, R Zhu, Y Zhang, SQ Li, HW Wang… - …, 2019 - academic.oup.com
Background Completing a genome is an important goal of genome assembly. However,
many assemblies, including reference assemblies, are unfinished and have a number of …
many assemblies, including reference assemblies, are unfinished and have a number of …
Reference-guided de novo assembly approach improves genome reconstruction for related species
HEL Lischer, KK Shimizu - BMC bioinformatics, 2017 - Springer
Background The development of next-generation sequencing has made it possible to
sequence whole genomes at a relatively low cost. However, de novo genome assemblies …
sequence whole genomes at a relatively low cost. However, de novo genome assemblies …
LRScaf: improving draft genomes using long noisy reads
Background The advent of third-generation sequencing (TGS) technologies opens the door
to improve genome assembly. Long reads are promising for enhancing the quality of …
to improve genome assembly. Long reads are promising for enhancing the quality of …
Long-read sequence assembly: a technical evaluation in barley
Sequence assembly of large and repeat-rich plant genomes has been challenging,
requiring substantial computational resources and often several complementary sequence …
requiring substantial computational resources and often several complementary sequence …