Genome reduction boosts heterologous gene expression in Pseudomonas putida

S Lieder, PI Nikel, V de Lorenzo, R Takors - Microbial cell factories, 2015 - Springer
Background The implementation of novel platform organisms to be used as microbial cell
factories in industrial applications is currently the subject of intense research. Ongoing …

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

E Martínez-García, PI Nikel, T Aparicio… - Microbial cell …, 2014 - Springer
Background Because of its adaptability to sites polluted with toxic chemicals, the model soil
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …

Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida

KR Choi, JS Cho, IJ Cho, D Park, SY Lee - Metabolic engineering, 2018 - Elsevier
Pseudomonas putida has gained much interest among metabolic engineers as a workhorse
for producing valuable natural products. While a few gene knockout tools for P. putida have …

Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications

P Liang, Y Zhang, B Xu, Y Zhao, X Liu, W Gao… - Microbial Cell …, 2020 - Springer
Background Genome streamlining is a feasible strategy for constructing an optimum
microbial chassis for synthetic biology applications. Genomic islands (GIs) are usually …

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

TB Cook, JM Rand, W Nurani… - Journal of Industrial …, 2018 - academic.oup.com
Pseudomonas putida is a promising bacterial host for producing natural products, such as
polyketides and nonribosomal peptides. In these types of projects, researchers need a …

Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome‐wide mutant library

MA Molina‐Henares, J De La Torre… - Environmental …, 2010 - Wiley Online Library
In silico models for Pseudomonas putida KT2440 metabolism predict 68 genes to be
essential for growth on minimal medium. In this study a genome‐wide collection of single …

[HTML][HTML] Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida

DC Volke, L Friis, NT Wirth, J Turlin, PI Nikel - Metabolic engineering …, 2020 - Elsevier
Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

S Zobel, I Benedetti, L Eisenbach… - ACS synthetic …, 2015 - ACS Publications
The soil bacterium Pseudomonas putida is increasingly attracting considerable interest as a
platform for advanced metabolic engineering through synthetic biology approaches …

Molecular tools and emerging strategies for deep genetic/genomic refactoring of Pseudomonas

E Martinez-Garcia, V de Lorenzo - Current opinion in biotechnology, 2017 - Elsevier
Highlights•Pseudomonas species are well suited as a platform for industrial and
environmental biotechnology.•A range of Synthetic Biology methods for genetic refactoring …