Genome reduction boosts heterologous gene expression in Pseudomonas putida
S Lieder, PI Nikel, V de Lorenzo, R Takors - Microbial cell factories, 2015 - Springer
Background The implementation of novel platform organisms to be used as microbial cell
factories in industrial applications is currently the subject of intense research. Ongoing …
factories in industrial applications is currently the subject of intense research. Ongoing …
Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression
Background Because of its adaptability to sites polluted with toxic chemicals, the model soil
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …
Industrial biotechnology of Pseudomonas putida: advances and prospects
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …
Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida
Pseudomonas putida has gained much interest among metabolic engineers as a workhorse
for producing valuable natural products. While a few gene knockout tools for P. putida have …
for producing valuable natural products. While a few gene knockout tools for P. putida have …
Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications
P Liang, Y Zhang, B Xu, Y Zhao, X Liu, W Gao… - Microbial Cell …, 2020 - Springer
Background Genome streamlining is a feasible strategy for constructing an optimum
microbial chassis for synthetic biology applications. Genomic islands (GIs) are usually …
microbial chassis for synthetic biology applications. Genomic islands (GIs) are usually …
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida
TB Cook, JM Rand, W Nurani… - Journal of Industrial …, 2018 - academic.oup.com
Pseudomonas putida is a promising bacterial host for producing natural products, such as
polyketides and nonribosomal peptides. In these types of projects, researchers need a …
polyketides and nonribosomal peptides. In these types of projects, researchers need a …
Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome‐wide mutant library
MA Molina‐Henares, J De La Torre… - Environmental …, 2010 - Wiley Online Library
In silico models for Pseudomonas putida KT2440 metabolism predict 68 genes to be
essential for growth on minimal medium. In this study a genome‐wide collection of single …
essential for growth on minimal medium. In this study a genome‐wide collection of single …
[HTML][HTML] Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida
Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …
Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida
S Zobel, I Benedetti, L Eisenbach… - ACS synthetic …, 2015 - ACS Publications
The soil bacterium Pseudomonas putida is increasingly attracting considerable interest as a
platform for advanced metabolic engineering through synthetic biology approaches …
platform for advanced metabolic engineering through synthetic biology approaches …
Molecular tools and emerging strategies for deep genetic/genomic refactoring of Pseudomonas
E Martinez-Garcia, V de Lorenzo - Current opinion in biotechnology, 2017 - Elsevier
Highlights•Pseudomonas species are well suited as a platform for industrial and
environmental biotechnology.•A range of Synthetic Biology methods for genetic refactoring …
environmental biotechnology.•A range of Synthetic Biology methods for genetic refactoring …
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