Despite the importance of DNA methylation in health and disease, technologies to readily
manipulate methylation of specific sequences for functional analysis and therapeutic …

Comprehensive studies have shown that DNA methylation plays vital roles in both loss of
pluripotency and governance of the transcriptome during embryogenesis and subsequent …

Mammalian genomes exhibit complex patterns of gene expression regulated, in part, by
DNA methylation. The advent of engineered DNA methyltransferases (MTases) to target …

DNA methylation plays a critical role in the regulation and maintenance of cell-type specific
transcriptional programs. Targeted epigenome editing is an emerging technology to …

Advances in sequencing technology allow researchers to map genome-wide changes in
DNA methylation in development and disease. However, there is a lack of experimental …

Mammalian DNA methylation is a critical epigenetic mechanism orchestrating gene
expression networks in many biological processes. However, investigation of the functions …

Background DNA methylation has widespread effects on gene expression during
development. However, our ability to assign specific function to regions of DNA methylation …

Detection of DNA methylation in the genome has been possible for decades; however, the
ability to deliberately and specifically manipulate local DNA methylation states in the …

DNA methylation is a key epigenetic modification involved in regulating gene expression
and maintaining genomic integrity. Here we inactivated all three catalytically active DNA …

Background Fusion of DNA methyltransferase domains to the nuclease-deficient clustered
regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has …