[PDF][PDF] Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion

MIM Cerda, R Gray, DP Higgins - academia.edu
Measurement of cytokine gene expression by reverse transcription quantitative polymerase
chain reaction (RT-qPCR) is used widely to assess the immune system of animals and to …

[HTML][HTML] Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals

MIM Cerda, R Gray, DP Higgins - PeerJ, 2020 - peerj.com
Measurement of cytokine gene expression by reverse transcription quantitative polymerase
chain reaction (RT-qPCR) is used widely to assess the immune system of animals and to …

[HTML][HTML] Developing immune profiles of endangered Australian sea lion (Neophoca cinerea) pups within the context of endemic hookworm (Uncinaria sanguinis) …

MI Meza Cerda, R Gray, PC Thomson… - Frontiers in Veterinary …, 2022 - frontiersin.org
As a top predator, the endangered Australian sea lion (Neophoca cinerea) is a sentinel of
ecosystem change, where population trends can reflect broader shifts in the marine …

[HTML][HTML] Monitoring bottlenose dolphin leukocyte cytokine mRNA responsiveness by qPCR

AR Hofstetter, KC Eberle, SK Venn-Watson… - PloS one, 2017 - journals.plos.org
Both veterinarians caring for dolphins in managed populations and researchers monitoring
wild populations use blood-based diagnostics to monitor bottlenose dolphin (Tursiops …

Immunophenotypes of Australian Sea Lion Pups (Neophoca cinerea) Developing in the Context of Hookworm Infection (Uncinaria sanguinis)

MI Meza Cerda - 2021 - ses.library.usyd.edu.au
Pinnipeds are exposed to anthropogenic and environmental stressors, including disease,
and these can, in turn, affect their immune system, health, and viability. The endemic …

[HTML][HTML] Expression profiles of the immune genes CD4, CD8β, IFNγ, IL-4, IL-6 and IL-10 in mitogen-stimulated koala lymphocytes (Phascolarctos cinereus) by qRT …

IE Maher, JE Griffith, Q Lau, T Reeves, DP Higgins - PeerJ, 2014 - peerj.com
Investigation of the immune response of the koala (Phascolarctos cinereus) is needed
urgently, but has been limited by scarcity of species-specific reagents and methods for this …

Development of a real-time quantitative RT-PCR (Taqman®) assay to measure cytokine profiles in California sea lions (Zalophus californianus) and Bottlenose …

C Funke, A Aldridge, C Leutenegger, BR Smith… - … Association for Aquatic …, 2002 - vin.com
Cytokines are a group of proteins that mediate complex immune cell interactions and
regulate the development of effector immune responses. The accurate measurement of …

[HTML][HTML] Development and testing of species-specific ELISA assays to measure IFN-γ and TNF-α in bottlenose dolphins (Tursiops truncatus)

KC Eberle, SK Venn-Watson, ED Jensen, J LaBresh… - Plos one, 2018 - journals.plos.org
Monitoring the immune status of cetaceans is important for a variety of health conditions.
Assays to quantify cytokines, especially pro-inflammatory cytokines, could be employed, in …

Development of a cytokine gene expression assay for the relative quantification of the African elephant (Loxodonta africana) cell-mediated immune responses

CR de Waal, L Kleynhans, SDC Parsons, WJ Goosen… - Cytokine, 2021 - Elsevier
Immunological assays are the basis for many diagnostic tests for infectious diseases in
animals and humans. Application in wildlife species, including the African elephant …

Development of reverse-transcriptase quantitative PCR assays for detection of the cytokines IL-1β, TNF-α, and IL-10 in chelonians

JM Rayl, JFX Wellehan Jr, D Bunick, MC Allender - Cytokine, 2019 - Elsevier
In response to viral pathogens, a host releases pro-inflammatory cytokines such as
interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) and anti-inflammatory …