Real-time, or quantitative PCR, is a valuable technique useful in bacteriophage research to
quantify the abundance of phage or host gene transcripts. It can be used during the infection …

Bacteriophages manipulate bacterial gene expression in order to express their own genes
or influence bacterial metabolism. Gene expression can be studied using real-time PCR or …

The plaque assay is the traditional method for the quantification of bacteriophage,
particularly for λ cloning vectors. Unfortunately, this technique is fraught with procedural …

PCR is a quick and effective way of identifying the presence and 'affiliation'of
bacteriophages, or phage-encoded genes from environmental samples, bacterial cells or …

The infectious cycles of viruses are known to cause dramatic changes to host cell function.
The development of microarray technology has provided means to monitor host cell …

Bacteriophages represent a promising alternative for controlling pathogenic bacteria. They
are ubiquitous in the environment, and their isolation is usually simple and fast. However …

Diagnostic systems based on reverse transcription (RT)-PCR are widely used for the
detection of viral genomes in different human specimens. The application of internal controls …

Whole genome wide analysis of transcription using RNA-Seq methods is a powerful way to
elucidate differential expression of gene features in bacteria across different conditions as …

The protocol in this unit describes the steps for gene expression analysis of Haemophilus
influenzae in broth culture. Steps for hot acid phenol RNA extraction, RNA cleanup …

Bacteriophage φμ-4 was purified from lysates by isoelectric precipitation and CsCl gradient
centrifugation. This phage displays an Escherichia coli phage lambda-like appearance in …