Gene activation by the CRISPR/Cas9 system has the potential to enable new approaches to
science and medicine, but the technology must be enhanced to robustly control cell …

Overexpression of exogenous fate-specifying transcription factors can directly reprogram
differentiated somatic cells to target cell types. Here, we show that similar reprogramming …

CRISPR/Cas9 protein fused to transactivation domains can be used to control gene
expression in human cells. In this study, we demonstrate that a dCas9 fusion with repeats of …

CRISPR-Cas9-based gene activation (CRISPRa) is an attractive tool for cellular
reprogramming applications due to its high multiplexing capacity and direct targeting of …

The speed of reprogramming technologies evolution is rising dramatically in modern
science. Both the scientific community and health workers depend on such developments …

Direct cell reprogramming, also called transdifferentiation, allows for the reprogramming of
one somatic cell type directly into another, without the need to transition through an induced …

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic
potential. Here, we review CRISPR-Cas systems and their latest developments with an …

As genome engineering advances cell-based therapies, a versatile approach to introducing
both CRISPR-Cas9 ribonucleoproteins (RNPs) and therapeutic transgenes into specific …

Precise temporal control of gene expression or deletion is critical for elucidating gene
function in biological systems. However, the establishment of human pluripotent stem cell …

All science takes inspiration from nature, but nowhere is this more true than in biology,
where some of the most powerful tools available to researchers are derived from natural …