Highlights•Advances in CRISPR-Cas9 aid tool development and multiplexed yeast
engineering.•New catalogue of promoters, trans-acting factors and terminators advance …

Saccharomyces cerevisiae is one of the most extensively used biosynthetic systems for the
production of diverse bioproducts, especially biotherapeutics and recombinant proteins …

Although only 6 years old, the CRISPR system has blossomed into a tool for rapid, on-
demand genome engineering and gene regulation in Saccharomyces cerevisiae. In this …

Saccharomyces cerevisiae has been for a long time a common model for fundamental
biological studies and a popular biotechnological engineering platform to produce …

Due to the rapidly increasing sequence information on gene variants generated by evolution
and the improved abilities to engineer novel biological activities, microbial cells can be …

The yeast S. cerevisiae serves as a model organism for many decades. Numerous
molecular tools have been developed throughout the years to engineer its genome …

Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced
short palindromic repeats (CRISPR) system has been increasingly used in the design and …

Saccharomyces cerevisiae is an important model eukaryotic microorganism and widely
applied in fundamental research and the production of various chemicals. Its ability to …

Genome-scale engineering is indispensable in understanding and engineering
microorganisms, but the current tools are mainly limited to bacterial systems. Here we report …