Assessment of SCAR markers to design real‐time PCR primers for rhizosphere quantification of Azospirillum brasilense phytostimulatory inoculants of maize

O Couillerot, MA Poirier… - Journal of Applied …, 2010 - academic.oup.com
Aims: To assess the applicability of sequence characterized amplified region (SCAR)
markers obtained from BOX, ERIC and RAPD fragments to design primers for real‐time PCR …

Detection of Azospirillum brasilense by qPCR throughout a maize field trial

S Urrea-Valencia, RM Etto, WY Takahashi, EF Caires… - Applied Soil …, 2021 - Elsevier
Although Azospirillum brasilense is used in many inoculant formulations, information of its
prevalence under field conditions is still scarce. In this work, we inoculated A. brasilense …

Applicability of the 16S–23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil

E Baudoin, O Couillerot, S Spaepen… - Journal of Applied …, 2010 - academic.oup.com
Aims: To assess the applicability of the 16S–23S rDNA internal spacer regions (ISR) as
targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth …

Accessing inoculation methods of maize and wheat with Azospirillum brasilense

J Fukami, MA Nogueira, RS Araujo, M Hungria - AMB express, 2016 - Springer
The utilization of inoculants containing Azospirillum is becoming more popular due to
increasing reports of expressive gains in grain yields. However, incompatibility with …

Inoculation with the plant-growth-promoting rhizobacterium Azospirillum brasilense causes little disturbance in the rhizosphere and rhizoplane of maize (Zea mays)

Y Herschkovitz, A Lerner, Y Davidov, M Rothballer… - Microbial Ecology, 2005 - Springer
Inoculation with Azospirillum brasilense exerts beneficial effects on plant growth and crop
yields. In this study, a comparative analysis of maize (Zea mays) root inoculated or not …

Quantification of Azospirillum brasilense FP2 bacteria in wheat roots by strain-specific quantitative PCR

MI Stets, SMC Alqueres, EM Souza… - Applied and …, 2015 - Am Soc Microbiol
Azospirillum is a rhizobacterial genus containing plant growth-promoting species associated
with different crops worldwide. Azospirillum brasilense strains exhibit a growth-promoting …

Real-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions

IC Soares, RS Pacheco, CGN da Silva, RS Santos… - Plant and Soil, 2021 - Springer
Purpose Bacterial quantification by qPCR is considered the gold standard for microbial
molecular diagnosis. However, a fundamental pre-requisite in this methodology is the …

Azospirillum brasilense viable cells enumeration using propidium monoazide-quantitative PCR

ET da Cunha, AM Pedrolo, F Paludo, MC Scariot… - Archives of …, 2020 - Springer
Azospirillum brasilense is a plant growth promoting bacteria used as an inoculant in diverse
crops. Accurate analytical methods are required to enumerate viable cells in inoculant …

Comparison of prominent Azospirillum strains in AzospirillumPseudomonasGlomus consortia for promotion of maize growth

O Couillerot, A Ramírez-Trujillo, V Walker… - Applied microbiology …, 2013 - Springer
Azospirillum are prominent plant growth-promoting rhizobacteria (PGPR) extensively used
as phytostimulatory crop inoculants, but only few studies are dealing with Azospirillum …

Development of a real-time PCR method to quantify the PGPR strain Azospirillum lipoferum CRT1 on maize seedlings

O Couillerot, ML Bouffaud, E Baudoin, D Muller… - Soil Biology and …, 2010 - Elsevier
Azospirillum lipoferum CRT1 is a promising phytostimulatory PGPR for maize, whose effect
on the plant is cell density-dependent. A nested PCR method is available for detection of the …