[HTML][HTML] Genome engineering in rice using Cas9 variants that recognize NG PAM sequences
K Hua, X Tao, P Han, R Wang, JK Zhu - Molecular plant, 2019 - cell.com
CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …
[HTML][HTML] Targeted base editing in rice with CRISPR/ScCas9 system
M Wang, Z Xu, G Gosavi, B Ren, Y Cao… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …
[HTML][HTML] A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants
CRISPR/Cas9 genome targeting systems have been applied to a variety of species.
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …
[HTML][HTML] Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
G Liang, H Zhang, D Lou, D Yu - Scientific reports, 2016 - nature.com
Abstract The CRISPR/Cas9-sgRNA system has been developed to mediate genome editing
and become a powerful tool for biological research. Employing the CRISPR/Cas9-sgRNA …
and become a powerful tool for biological research. Employing the CRISPR/Cas9-sgRNA …
[HTML][HTML] Application of the CRISPR–Cas system for efficient genome engineering in plants
Y Mao, H Zhang, N Xu, B Zhang, F Gou, JK Zhu - Molecular plant, 2013 - cell.com
Dear Editor, Recently, engineered endonucleases, such as Zinc-Finger Nucleases
(ZFNs)(Carroll, 2011), Transcription Activator-Like Effector Nucleases (TALENs)(Mahfouz et …
(ZFNs)(Carroll, 2011), Transcription Activator-Like Effector Nucleases (TALENs)(Mahfouz et …
PAM-less plant genome editing using a CRISPR–SpRY toolbox
Q Ren, S Sretenovic, S Liu, X Tang, L Huang, Y He… - Nature plants, 2021 - nature.com
The rapid development of the CRISPR–Cas9,–Cas12a and–Cas12b genome editing
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …
[HTML][HTML] Expanding the range of CRISPR/Cas9 genome editing in rice
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
[PDF][PDF] Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice
The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) system
has been widely used for a variety of applications, including targeted gene knockout, gene …
has been widely used for a variety of applications, including targeted gene knockout, gene …
[HTML][HTML] Expanding the scope of CRISPR/Cpf1-mediated genome editing in rice
The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
[HTML][HTML] Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention
Key message We present novel observations of high-specificity SpCas9 variants, sgRNA
expression strategies based on mutant sgRNA scaffold and tRNA processing system, and …
expression strategies based on mutant sgRNA scaffold and tRNA processing system, and …