Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring

Z Khosravizadeh, K Khodamoradi, Z Rashidi… - Journal of assisted …, 2022 - Springer
Despite the beneficial effects of sperm cryopreservation, increased reactive oxygen species
(ROS) production during this process can affect spermatozoon structure and function …

Routine cryopreservation of spermatozoa is safe—evidence from the DNA methylation pattern of nine spermatozoa genes

R Kläver, A Bleiziffer, K Redmann, C Mallidis… - Journal of assisted …, 2012 - Springer
Purpose Assess short-and mid-term impact of cryopreservation on DNA methylation status of
different genes in spermatozoa. Methods Semen samples from 10 healthy normozoospermic …

Spermatozoa cryopreservation alters pronuclear formation and zygotic DNA demethylation in mice

G Jia, X Fu, K Cheng, M Yue, B Jia, Y Hou, S Zhu - Theriogenology, 2015 - Elsevier
This study was conducted to investigate the effects of spermatozoa cryopreservation on DNA
demethylation in mouse zygotes. Global methylation was studied in zygotes fertilized with …

The effect of cryopreservation on DNA methylation patterns of the chromosome 15q11–q13 region in human spermatozoa

Z Khosravizadeh, G Hassanzadeh… - Cell and Tissue …, 2020 - Springer
Human sperm cryopreservation is a common technique which is used in assisted
reproductive technologies. Despite the existence of evidence supporting the production of …

Sperm cryopreservation: effects on chromatin structure

D Paoli, F Lombardo, A Lenzi, L Gandini - Genetic damage in human …, 2014 - Springer
Cryopreservation is a technique that can keep sperm alive indefinitely, enabling the
conservation of male fertility. It involves the cooling of semen samples and their storage at …

[HTML][HTML] Comparative epigenetic analysis of imprinting genes involved in fertility, in cryopreserved human sperms with rapid freezing versus vitrification methods

N Khosronezhad, V Hassanzadeh… - Cell Journal …, 2023 - ncbi.nlm.nih.gov
Objective: Choosing the optimal method for human sperm cryopreservation seems
necessary to reduce cryoinjury. The aim of this study is to compare two cryopreservation …

Cryopreservation of sperm: effects on chromatin and strategies to prevent them

D Paoli, M Pelloni, A Lenzi, F Lombardo - Genetic Damage in Human …, 2019 - Springer
Cryopreservation is a technique that can keep sperm alive indefinitely, enabling the
conservation of male fertility. It involves the cooling of semen samples and their storage at …

Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches

M Hezavehei, M Sharafi, HM Kouchesfahani… - Reproductive …, 2018 - Elsevier
The cryopreservation of spermatozoa was introduced in the 1960s as a route to fertility
preservation. Despite the extensive progress that has been made in this field, the biological …

[PDF][PDF] Sperm cryopreservation: principles and biology

Y Sharma, M Sharma - Journal of Infertility and Reproductive …, 2020 - researchgate.net
Cryopreservation is a widely used method to preserve sperm prior to any cytotoxic therapy
or testicular surgical intervention to be used later for assisted conception. Despite of multiple …

[HTML][HTML] Effects of cryopreservation on human sperm deoxyribonucleic acid integrity

N Zribi, NF Chakroun, H El Euch, J Gargouri… - Fertility and sterility, 2010 - Elsevier
OBJECTIVE: To evaluate the effect of cryopreservation on sperm motility and viability and to
assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation …