Repurposing the Endogenous Type IE CRISPR/Cas System for Gene Repression in Gluconobacter oxydans WSH-003
Z Qin, Y Yang, S Yu, L Liu, Y Chen, J Chen… - ACS synthetic …, 2021 - ACS Publications
Gluconobacter oxydans is well-known for its incomplete oxidizing capacity and has been
widely applied in industrial production. However, genetic tools in G. oxydans are still scarce …
widely applied in industrial production. However, genetic tools in G. oxydans are still scarce …
Novel prokaryotic CRISPR-Cas12a-based tool for programmable transcriptional activation and repression
C Schilling, MAG Koffas, V Sieber… - ACS Synthetic …, 2020 - ACS Publications
Transcriptional perturbation using inactivated CRISPR-nucleases (dCas) is a common
method in eukaryotic organisms. While rare examples of dCas9-based tools for prokaryotes …
method in eukaryotic organisms. While rare examples of dCas9-based tools for prokaryotes …
[HTML][HTML] RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain
Background The construction of microbial cell factories requires cost-effective and rapid
strain development through metabolic engineering. Recently, RNA-guided CRISPR …
strain development through metabolic engineering. Recently, RNA-guided CRISPR …
A high-efficacy CRISPR interference system for gene function discovery in Zymomonas mobilis
Zymomonas mobilis is a promising biofuel producer due to its high alcohol tolerance and
streamlined metabolism that efficiently converts sugar to ethanol. Z. mobilis genes are poorly …
streamlined metabolism that efficiently converts sugar to ethanol. Z. mobilis genes are poorly …
CRISPR-based metabolic engineering in non-model microorganisms
Highlights•Non-model microorganisms serve as promising industrial chassis for
bioproduction.•CRISPR-based genetic modifications enable desired production …
bioproduction.•CRISPR-based genetic modifications enable desired production …
Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production
Abstract Although CRISPR-Cas9/Cpf1 have been employed as powerful genome
engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into …
engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into …
Next generation prokaryotic engineering: the CRISPR-Cas toolkit
The increasing demand for environmentally friendly production processes of green
chemicals and fuels has stimulated research in microbial metabolic engineering. CRISPR …
chemicals and fuels has stimulated research in microbial metabolic engineering. CRISPR …
CRISPR interference‐mediated gene regulation in Pseudomonas putida KT2440
SK Kim, PK Yoon, SJ Kim, SG Woo, E Rha… - Microbial …, 2020 - Wiley Online Library
Targeted gene regulation is indispensable for reprogramming a cellular network to modulate
a microbial phenotype. Here, we adopted the type II CRISPR interference (CRISPR i) system …
a microbial phenotype. Here, we adopted the type II CRISPR interference (CRISPR i) system …
[HTML][HTML] Applications of CRISPR/Cas system to bacterial metabolic engineering
The clustered regularly interspaced short palindromic repeats/CRISPR-associated
(CRISPR/Cas) adaptive immune system has been extensively used for gene editing …
(CRISPR/Cas) adaptive immune system has been extensively used for gene editing …
[HTML][HTML] Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum
M Li, J Chen, Y Wang, J Liu, J Huang… - … in Bioengineering and …, 2020 - frontiersin.org
Corynebacterium glutamicum is an important workhorse for industrial production of
diversiform bioproducts. Multiplex control of metabolic pathway genes is crucial for …
diversiform bioproducts. Multiplex control of metabolic pathway genes is crucial for …