New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i
J Villette, F Lecourieux, E Bastiancig, MC Héloir… - Plant Methods, 2024 - Springer
Background For ten years, CRISPR/cas9 system has become a very useful tool for obtaining
site-specific mutations on targeted genes in many plant organisms. This technology opens …
site-specific mutations on targeted genes in many plant organisms. This technology opens …
Efficiency optimization of CRISPR/Cas9-mediated targeted mutagenesis in grape
F Ren, C Ren, Z Zhang, W Duan… - Frontiers in plant …, 2019 - frontiersin.org
Clustered regularly interspersed short palindromic repeats (CRISPR)/Cas system is an
efficient targeted genome editing method. Although CRISPR/Cas9-mediated mutagenesis …
efficient targeted genome editing method. Although CRISPR/Cas9-mediated mutagenesis …
CRISPR/Cas9‐mediated efficient targeted mutagenesis in grape in the first generation
X Wang, M Tu, D Wang, J Liu, Y Li, Z Li… - Plant biotechnology …, 2018 - Wiley Online Library
The clustered regularly interspaced short palindromic repeats‐associated protein 9
(CRISPR/Cas9) system is a powerful tool for editing plant genomes. Efficient genome editing …
(CRISPR/Cas9) system is a powerful tool for editing plant genomes. Efficient genome editing …
Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine
X Wang, M Tu, Y Wang, W Yin, Y Zhang… - Horticulture …, 2021 - academic.oup.com
The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein
9 (Cas9) system is a powerful tool for targeted genome editing, with applications that include …
9 (Cas9) system is a powerful tool for targeted genome editing, with applications that include …
CRISPR/Cas9-mediated targeted mutagenesis in grape
I Nakajima, Y Ban, A Azuma, N Onoue, T Moriguchi… - PLoS …, 2017 - journals.plos.org
RNA-guided genome editing using the CRISPR/Cas9 CRISPR (clustered regularly
interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) system has …
interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) system has …
DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration
S Najafi, E Bertini, E D'Incà, M Fasoli… - Horticulture …, 2023 - academic.oup.com
CRISPR/Cas9 genome editing technology can overcome many limitations of traditional
breeding, offering enormous potential for crop improvement and food production. Although …
breeding, offering enormous potential for crop improvement and food production. Although …
Identification of genomic sites for CRISPR/Cas9-based genome editing in the Vitis vinifera genome
Y Wang, X Liu, C Ren, GY Zhong, L Yang, S Li… - BMC plant biology, 2016 - Springer
Background CRISPR/Cas9 has been recently demonstrated as an effective and popular
genome editing tool for modifying genomes of humans, animals, microorganisms, and …
genome editing tool for modifying genomes of humans, animals, microorganisms, and …
Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters
C Ren, Y Liu, Y Guo, W Duan, P Fan, S Li… - Horticulture …, 2021 - academic.oup.com
The efficacy of the CRISPR/Cas9 system in grapevine (Vitis vinifera L.) has been
documented, but the optimization of this system, as well as CRISPR/Cas9-mediated …
documented, but the optimization of this system, as well as CRISPR/Cas9-mediated …
[HTML][HTML] CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects
C Ren, Y Lin, Z Liang - Fruit Research, 2022 - maxapress.com
The development of clustered regularly interspaced short palindromic repeats/CRISPR
associated protein 9 (CRISPR/Cas9) system has revolutionized genome editing and plant …
associated protein 9 (CRISPR/Cas9) system has revolutionized genome editing and plant …
CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)
C Ren, X Liu, Z Zhang, Y Wang, W Duan, S Li… - Scientific reports, 2016 - nature.com
The type II clustered regularly interspaced short palindromic repeats/CRISPR-associated
protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in …
protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in …