PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin
B Gust, GL Challis, K Fowler… - Proceedings of the …, 2003 - National Acad Sciences
Streptomycetes are high G+ C Gram-positive, antibiotic-producing, mycelial soil bacteria.
The 8.7-Mb Streptomyces coelicolor genome was previously sequenced by using an …
The 8.7-Mb Streptomyces coelicolor genome was previously sequenced by using an …
Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase
DE Cane, X He, S Kobayashi, S Ōmura… - The Journal of …, 2006 - nature.com
Geosmin (1) is responsible for the characteristic odor of moist soil. The Gram-positive soil
bacterium Streptomyces avermitilis produces geosmin (1) as well as its precursor …
bacterium Streptomyces avermitilis produces geosmin (1) as well as its precursor …
Biosynthesis of the earthy odorant geosmin by a bifunctional Streptomyces coelicolor enzyme
J Jiang, X He, DE Cane - Nature Chemical Biology, 2007 - nature.com
Geosmin (1) is responsible for the characteristic odor of moist soil, as well as off-flavors in
drinking water and foodstuffs,. Geosmin is generated from farnesyl diphosphate (FPP, 2) by …
drinking water and foodstuffs,. Geosmin is generated from farnesyl diphosphate (FPP, 2) by …
Geosmin Biosynthesis. Streptomyces coelicolor Germacradienol/Germacrene D Synthase Converts Farnesyl Diphosphate to Geosmin
J Jiang, X He, DE Cane - Journal of the American Chemical …, 2006 - ACS Publications
Geosmin is responsible for the characteristic odor of moist soil. Incubation of recombinant
germacradienol synthase, encoded by the SCO6073 (SC9B1. 20) gene of the Gram-positive …
germacradienol synthase, encoded by the SCO6073 (SC9B1. 20) gene of the Gram-positive …
Geosmin biosynthesis. Mechanism of the fragmentation− rearrangement in the conversion of germacradienol to geosmin
J Jiang, DE Cane - Journal of the American Chemical Society, 2008 - ACS Publications
Geosmin (1) is responsible for the characteristic odor of moist soil. Incubation of farnesyl
diphosphate (2, FPP) with recombinant Streptomyces coelicolor germacradienol/geosmin …
diphosphate (2, FPP) with recombinant Streptomyces coelicolor germacradienol/geosmin …
Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis
The PCR has been used to amplify a 2,181-bp ORF from Streptomyces coelicolor A3 (2),
designated SC9B1. 20 (= SCO6073), encoding a protein of 726 amino acids and showing …
designated SC9B1. 20 (= SCO6073), encoding a protein of 726 amino acids and showing …
Biochemistry and Molecular Genetics of the Biosynthesis of the Earthy Odorant Methylisoborneol in Streptomyces coelicolor
CM Wang, DE Cane - Journal of the American Chemical Society, 2008 - ACS Publications
Methylisoborneol (2) is a volatile organic compound produced by a wide variety of
Actinomycete soil organisms, myxobacteria, and cyanobacteria. It has an unusually low odor …
Actinomycete soil organisms, myxobacteria, and cyanobacteria. It has an unusually low odor …
Genome Mining in Streptomyces coelicolor: Molecular Cloning and Characterization of a New Sesquiterpene Synthase
X Lin, R Hopson, DE Cane - Journal of the American Chemical …, 2006 - ACS Publications
The terpene synthase encoded by the SCO5222 (SC7E4. 19) gene of Streptomyces
coelicolor was cloned by PCR and expressed in Escherichia coli as an N-terminal-His6-tag …
coelicolor was cloned by PCR and expressed in Escherichia coli as an N-terminal-His6-tag …
Developmentally regulated volatiles geosmin and 2-methylisoborneol attract a soil arthropod to Streptomyces bacteria promoting spore dispersal
Volatile compounds emitted by bacteria are often sensed by other organisms as odours, but
their ecological roles are poorly understood,. Well-known examples are the soil-smelling …
their ecological roles are poorly understood,. Well-known examples are the soil-smelling …
Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters
JP Gomez‐Escribano, MJ Bibb - Microbial biotechnology, 2011 - Wiley Online Library
We have constructed derivatives of Streptomyces coelicolor M145 as hosts for the
heterologous expression of secondary metabolite gene clusters. To remove potentially …
heterologous expression of secondary metabolite gene clusters. To remove potentially …