Advances in cryogenic electron microscopy (cryoEM) single particle analysis have
revolutionized structural biology by facilitating the in vitro determination of atomic-and near …

Cryo-electron microscopy (cryo-EM) is an impactful methodology for three-dimensional (3D)
structural determination of macromolecular complexes. While single particle EM gained …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Cryo-electron tomography (cryo-ET) is an emerging technology for the 3D visualization of
structural organizations and interactions of subcellular components at near-native state and …

Highlights•Electron cryo-tomography (cryoET) reveals structures in a near-native
state.•Recent technological advances have increased the information obtainable by …

Cryo-electron microscopy (cryo-EM) can be used to elucidate the 3D structure of
macromolecular complexes. Driven by technological breakthroughs in electron-microscope …

Over the past decade, technical and methodological improvements in cryogenic electron
microscopy (cryo-EM) single-particle analysis have enabled routine high-resolution …

Electron cryotomography is a rapidly evolving method for imaging macromolecules directly
within the native environment of cells and tissues. Combined with sub-tomogram averaging …

Recent advances in single-particle cryogenic electron microscopy (cryo-EM) have enabled
the structural determination of numerous protein assemblies at high resolution, yielding …

Combining diverse experimental structural and interactomic methods allows for the
construction of comprehensible molecular encyclopedias of biological systems. Typically …