Fluorescence lifetime imaging–techniques and applications

W Becker - Journal of microscopy, 2012 - Wiley Online Library
Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Time-domain fluorescence lifetime imaging applied to biological tissue

D Elson, J Requejo-Isidro, I Munro, F Reavell… - Photochemical & …, 2004 - Springer
Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide
information, not only concerning the localisation of specific fluorophores, but also about the …

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Fluorescence lifetime imaging microscopy

CW Chang, D Sud, MA Mycek - Methods in cell biology, 2007 - Elsevier
Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially
resolved images of fluorophore lifetime (the property describing how rapidly fluorescence …

Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications

XF Wang, A Periasamy, B Herman… - Critical Reviews in …, 1992 - Taylor & Francis
The new and novel techniques of fluorescence lifetime imaging (FLI)∗∗ and fluorescence
lifetime imaging microscopy (FLIM) provide the investigator with the capacity to quantitate …

Fluorescence lifetime imaging microscopy (FLIM)

EB van Munster, TWJ Gadella - Microscopy Techniques: -/-, 2005 - Springer
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial
distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems …

A novel fluorescence lifetime imaging system that optimizes photon efficiency

RA Colyer, C Lee, E Gratton - Microscopy research and …, 2008 - Wiley Online Library
Fluorescence lifetime imaging (FLIM) is a powerful microscopy technique for providing
contrast of biological and other systems by differences in molecular species or their …

Wide-field fluorescence lifetime imaging of single molecules

N Oleksiievets, JC Thiele, A Weber… - The Journal of …, 2020 - ACS Publications
Fluorescence lifetime imaging (FLIM) has become an important microscopy technique in
bioimaging. The two most important of its applications are lifetime-multiplexing for imaging …

Imaging proteins in vivo using fluorescence lifetime microscopy

F Festy, SM Ameer-Beg, T Ng, K Suhling - Molecular Biosystems, 2007 - pubs.rsc.org
Imaging proteins in vivo using fluorescence lifetime microscopy - Molecular BioSystems (RSC
Publishing) DOI:10.1039/B617204K Royal Society of Chemistry View PDF VersionPrevious …