A general method for affinity-based proteomic profiling of exo -α-glycosidases - Chemical
Communications (RSC Publishing) DOI:10.1039/C1CC10308C Royal Society of Chemistry View …

Tunable Förster resonance energy transfer (FRET)-quenched substrates are useful for
monitoring the activity of various enzymes within their relevant physiological environments …

The assignment of protein glycosylation sites and their microheterogeneities are of
biological importance, yet such characterization is still considered to be analytically very …

The most commonly employed glycosidase assays rely on bulky ultraviolet or fluorescent
tags at the anomeric position in potential carbohydrate substrates, thereby limiting the utility …

Exoglycosidase digestion coupled with matrix-assisted laser desorption/ionization mass
spectrometry (MALDI-MS) is an effective technique for sequencing the N-linked …

New functional proteomics methods are required for targeting and identification of subsets of
a proteome in an activity‐based fashion. Glycosidases play critical roles in biology, yet a …

Characterization of mucin-type O-glycans linked to serine/threonine of glycoproteins is
technically challenging, in part, due to a lack of effective enzymatic tools that enable their …

We designed and synthesized hydrogen bond based probes 1–8 with the exception of
known glycosidase inhibition mechanisms, and aglycon specificity of 11 different sources of …

In-gel digestion of densely O-glycosylated proteins, an essential step in proteome analysis,
is often hampered by steric hindrance of the proteases. To overcome this technical problem …

Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic
reticulum (ER) and Golgi apparatus. Over 50% of mammalian cellular proteins are typically …