We have characterized, by electron probe microanalysis, rapidly frozen cultured rat islets at
the level of individual secretory granules. Elemental analysis of thin, dried cryosections …

A partially purified secretory granule fraction, isolated from rat islets of Langerhans by
differential centrifugation, was used for investigating the stability of the beta granules during …

β-Granules were prepared from micro-dissected pancreatic islets of obese–hyperglycaemic
mice. This fraction contained 60% of the insulin, 30% of the cytochrome oxidase, 16% of the …

The concentrations of Zn2+, Ca2+, Mg2+, Pi and adenine nucleotides were determined in
insulin-secretory granules prepared from a transplantable rat insulinoma. Differential and …

Earlier antimonate techniques for electron microscopic localization of sodium were applied
to isolated islets of Langerhans obtained by partial collagenase digestion of mouse and …

A comparative study of the pancreatic islets of Langerhans was undertaken in which
correlative thick-thin sectioning techniques for light and electron microscopy were used. This …

Secretory granule-enriched fractions prepared from isolated rat islets of Langerhans,
previously labeled in culture for 18 h with [3H] leucine, have been lysed and separated into …

A method has been devised for the isolation of a secretory granule fraction from isolated rat
islets of Langerhans. The islets were homogenized in buffered sucrose, and the …

The ultrastructure of the beta granules is distinctly different in the islets of the rat, dog, cat,
rabbit, guinea pig and chicken. The variations are so characteristic that each species can be …

We have obtained evidence by autoradiography and immunocytochemistry that mature
secretory granules of the pancreatic B-cell gain access to a lysosomal compartment …