Our understanding of how genotype controls phenotype is limited by the scale at which we
can precisely alter the genome and assess the phenotypic consequences of each …

A major challenge in genetics is to identify genetic variants driving natural phenotypic
variation. However, current methods of genetic mapping have limited resolution. To address …

We developed a CRISPR–Cas9-and homology-directed-repair-assisted genome-scale
engineering method named CHAnGE that can rapidly output tens of thousands of specific …

Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …

Genome editing tools such as CRISPR/Cas9 enable the rapid and precise manipulation of
genomes. CRISPR-based genome editing has greatly simplified the study of gene function …

INTRODUCTION The developmental path by which a fertilized egg gives rise to the cells of a
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …

Genome modification in budding yeast has been extremely successful largely due to its
highly efficient homology-directed DNA repair machinery. Several methods for modifying the …

To the Editor—The field of genome editing is advancing rapidly1, most recently exemplified
by the advent of base editors that enable changing single nucleotides in a predictable …

CRISPR-Cas9 and related technologies enable the efficient alteration of genomic DNA at
targeted positions and are widely used in applications ranging from individual gene …

Yeasts are used to produce a myriad of value-added compounds. Engineering yeasts into
cost-efficient cell factories is greatly facilitated by the availability of genome editing tools …