Simplified adenine base editors improve adenine base editing efficiency in rice

K Hua, X Tao, W Liang, Z Zhang… - Plant Biotechnology …, 2020 - Wiley Online Library
Adenine base editors (ABE s) have been exploited to introduce targeted adenine (A) to
guanine (G) base conversions in various plant genomes, including rice, wheat and …

PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants

J Tan, D Zeng, Y Zhao, Y Wang, T Liu… - Plant Biotechnology …, 2022 - Wiley Online Library
Adenine base editors (ABEs), which are generally engineered adenosine deaminases and
Cas variants, introduce site‐specific A‐to‐G mutations for agronomic trait improvement …

[HTML][HTML] High-efficiency and multiplex adenine base editing in plants using new TadA variants

D Yan, B Ren, L Liu, F Yan, S Li, G Wang, W Sun… - Molecular plant, 2021 - cell.com
Recently reported adenine base editors (ABEs) exhibit powerful potential for targeted gene
correction as well as developing gain-of-function mutants and novel germplasms for both …

[HTML][HTML] Adenine base editor incorporating the N-methylpurine DNA glycosylase MPGv3 enables efficient A-to-K base editing in rice

X Wu, B Ren, L Liu, S Qiu, P Li, F Yan, H Lin… - Plant …, 2023 - cell.com
Dear Editor, 31 Crop genetic diversity and elite agronomic traits are mainly caused by
genetic variants, 32 of which approximately half are single-nucleotide polymorphisms …

Expanding the base editing scope in rice by using Cas9 variants

K Hua, X Tao, JK Zhu - Plant Biotechnology Journal, 2019 - Wiley Online Library
Base editing is a novel genome editing strategy that enables irreversible base conversion at
target loci without the need for double stranded break induction or homology‐directed …

[HTML][HTML] CRISPR/Cas9-mediated adenine base editing in rice genome

LI Hao, QIN Ruiying, LIU Xiaoshuang, L Shengxiang… - Rice Science, 2019 - Elsevier
Precise base editing is highly desired in plant functional genomic research and crop
molecular breeding. In this study, we constructed a rice-codon optimized adenine base …

[HTML][HTML] Increasing cytosine base editing scope and efficiency with engineered Cas9-PmCDA1 fusions and the modified sgRNA in rice

Y Wu, W Xu, F Wang, S Zhao, F Feng, J Song… - Frontiers in …, 2019 - frontiersin.org
Base editors that do not require double-stranded DNA cleavage or homology-directed repair
enable higher efficiency and cleaner substitution of targeted single nucleotides in genomic …

[HTML][HTML] Precise A· T to G· C base editing in the rice genome

K Hua, X Tao, F Yuan, D Wang, JK Zhu - Molecular plant, 2018 - cell.com
Precise modification of eukaryotic genomes has been accomplished mainly through
homology-directed repair (HDR) of DNA double-strand breaks (DSBs)(Hess et al., 2017) …

[HTML][HTML] Efficient C‐to‐G editing in rice using an optimized base editor

Y Tian, R Shen, Z Li, Q Yao, X Zhang… - Plant Biotechnology …, 2022 - ncbi.nlm.nih.gov
Cytosine and adenosine base editors (CBE and ABE) have been vigorously developed in
plants, but the base conversion types are limited (Ren et al., 2018, 2021; Xu et al., 2021). A …

[HTML][HTML] A precise and efficient adenine base editor

T Tu, Z Song, X Liu, S Wang, X He, H Xi, J Wang… - Molecular Therapy, 2022 - cell.com
Adenine base editors (ABEs) are novel genome-editing tools, and their activity has been
greatly enhanced by eight additional mutations, thus named ABE8e. However, elevated …