Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens
BJ Des Soye, SR Davidson, MT Weinstock… - ACS synthetic …, 2018 - ACS Publications
A new wave of interest in cell-free protein synthesis (CFPS) systems has shown their utility
for producing proteins at high titers, establishing genetic regulatory element libraries (eg …
for producing proteins at high titers, establishing genetic regulatory element libraries (eg …
Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens
J Failmezger, S Scholz, B Blombach… - Frontiers in …, 2018 - frontiersin.org
Vibrio natriegens constitutes one of the fastest-growing nonpathogenic bacteria and a
potential novel workhorse for many biotechnological applications. Here, we report the …
potential novel workhorse for many biotechnological applications. Here, we report the …
Establishing a Cell-Free Vibrio natriegens Expression System
The fast growing bacterium Vibrio natriegens is an emerging microbial host for
biotechnology. Harnessing its productive cellular components may offer a compelling …
biotechnology. Harnessing its productive cellular components may offer a compelling …
Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology
Over the last 50 years, Cell-Free Protein Synthesis (CFPS) has emerged as a powerful
technology to harness the transcriptional and translational capacity of cells within a test tube …
technology to harness the transcriptional and translational capacity of cells within a test tube …
High-throughput preparation methods of crude extract for robust cell-free protein synthesis
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …
technology platform for high-throughput protein production and genetic part characterization …
Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications
WQ Liu, L Zhang, M Chen, J Li - Biochemical Engineering Journal, 2019 - Elsevier
Cell-free protein synthesis (CFPS) is a technology for the production of proteins in vitro,
which has emerged as a robust platform for biotechnology and synthetic biology …
which has emerged as a robust platform for biotechnology and synthetic biology …
Establishing a Eukaryotic Pichia pastoris Cell-Free Protein Synthesis System
L Zhang, WQ Liu, J Li - Frontiers in Bioengineering and Biotechnology, 2020 - frontiersin.org
In recent years, cell-free protein synthesis (CFPS) systems have been used to synthesize
proteins, prototype genetic elements, manufacture chemicals, and diagnose diseases …
proteins, prototype genetic elements, manufacture chemicals, and diagnose diseases …
Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements
Cell-free protein synthesis (CFPS) systems enable the production of protein without the use
of living, intact cells. An emerging area of interest is to use CFPS systems to characterize …
of living, intact cells. An emerging area of interest is to use CFPS systems to characterize …
A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
Simplifying and streamlining Escherichia coli‐based cell‐free protein synthesis
WC Yang, KG Patel, HE Wong… - Biotechnology …, 2012 - Wiley Online Library
Escherichia coli cell‐free protein synthesis (CFPS) uses E. coli extracts to make active
proteins in vitro. The basic CFPS reaction mixture is comprised of four main reagent …
proteins in vitro. The basic CFPS reaction mixture is comprised of four main reagent …