Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens

BJ Des Soye, SR Davidson, MT Weinstock… - ACS synthetic …, 2018 - ACS Publications
A new wave of interest in cell-free protein synthesis (CFPS) systems has shown their utility
for producing proteins at high titers, establishing genetic regulatory element libraries (eg …

Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens

J Failmezger, S Scholz, B Blombach… - Frontiers in …, 2018 - frontiersin.org
Vibrio natriegens constitutes one of the fastest-growing nonpathogenic bacteria and a
potential novel workhorse for many biotechnological applications. Here, we report the …

Establishing a Cell-Free Vibrio natriegens Expression System

DJ Wiegand, HH Lee, N Ostrov… - ACS synthetic biology, 2018 - ACS Publications
The fast growing bacterium Vibrio natriegens is an emerging microbial host for
biotechnology. Harnessing its productive cellular components may offer a compelling …

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

MZ Levine, NE Gregorio, MC Jewett, KR Watts… - JoVE (Journal of …, 2019 - jove.com
Over the last 50 years, Cell-Free Protein Synthesis (CFPS) has emerged as a powerful
technology to harness the transcriptional and translational capacity of cells within a test tube …

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

YC Kwon, MC Jewett - Scientific reports, 2015 - nature.com
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …

Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications

WQ Liu, L Zhang, M Chen, J Li - Biochemical Engineering Journal, 2019 - Elsevier
Cell-free protein synthesis (CFPS) is a technology for the production of proteins in vitro,
which has emerged as a robust platform for biotechnology and synthetic biology …

Establishing a Eukaryotic Pichia pastoris Cell-Free Protein Synthesis System

L Zhang, WQ Liu, J Li - Frontiers in Bioengineering and Biotechnology, 2020 - frontiersin.org
In recent years, cell-free protein synthesis (CFPS) systems have been used to synthesize
proteins, prototype genetic elements, manufacture chemicals, and diagnose diseases …

Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements

H Wang, J Li, MC Jewett - Synthetic Biology, 2018 - academic.oup.com
Cell-free protein synthesis (CFPS) systems enable the production of protein without the use
of living, intact cells. An emerging area of interest is to use CFPS systems to characterize …

A user's guide to cell-free protein synthesis

NE Gregorio, MZ Levine, JP Oza - Methods and protocols, 2019 - mdpi.com
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …

Simplifying and streamlining Escherichia coli‐based cell‐free protein synthesis

WC Yang, KG Patel, HE Wong… - Biotechnology …, 2012 - Wiley Online Library
Escherichia coli cell‐free protein synthesis (CFPS) uses E. coli extracts to make active
proteins in vitro. The basic CFPS reaction mixture is comprised of four main reagent …