The temperature-inducible λ pL/pR-cI857 expression system has been widely used to
produce recombinant proteins (RPs), especially when it is necessary to avoid the addition of …

Background The temperature upshift has been widely used as an induction system to
produce recombinant proteins (RPs). However, thermoinduction could affect bacterial …

Recombinant protein (RP) production from Escherichia coli has been extensively studied to
find strategies for increasing product yields. The thermoinducible expression system is …

The overproduction of recombinant proteins in Escherichia coli leads to insoluble
aggregates of proteins called inclusion bodies (IBs). IBs are considered dynamic entities that …

Escherichia coli is used intensively for recombinant protein production, but one key
challenge with recombinant E. coli is the tendency of recombinant proteins to misfold and …

Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive
aggregates of misfolded protein, and thus should be avoided during recombinant protein …

Various fermentation strategies in industrial biotechnology are applied to produce
recombinant target proteins using Escherichia coli. These proteins are often expressed as …

Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …

The formation of inclusion bodies (IBs) is considered as an Achilles heel of heterologous
protein expression in bacterial hosts. Wide array of techniques has been developed to …

Production of recombinant proteins often interferes with the physiology of the host organism
by causing stress responses. In recombinant Escherichia coli, the cellular content of ColE1 …