The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously
reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin …

Firefly luciferin is a specialized metabolite restricted to fireflies (family Lampyridae) and other
select families of beetles (order Coleoptera). Firefly luciferin undergoes luciferase-catalyzed …

Coelenterazine (CTZ) is known as a light-emitting source for the bioluminescence reaction
in marine organisms. CTZ has two phenolic hydroxy groups at the C2-benzyl and C6-phenyl …

Genes from ancient families are sometimes involved in the convergent evolutionary origins
of similar traits, even across vast phylogenetic distances. Sulfotransferases are an ancient …

A sulfotransferase isolated from the Cyanobacterium Synechococcus 6301 was found to be
specific for 3′-phosphoadenosine-5′-phosphosulfate (PAPS). The molecular weight of …

The Renilla bioluminescent system in vivo is comprised of three proteins—the luciferase,
green-fluorescent protein, and coelenterazine-binding protein (CBP), previously called …

Renilla luciferase (RLUC) is a versatile tool for gene expression assays and in vivo
biosensor applications, but its catalytic mechanism remains to be elucidated. RLUC is …

Abstract A 3′-phosphoadenosine 5′-phosphosulfate (PAPS): desulfoglucosinolate
sulfotransferase (EC 2.8. 2-) was extensively purified from light-grown cress (Lepidium …

The cold-induced expression system in Escherichia coli is useful and we have applied this
system to prepare the coelenterazine-utilizing luciferases including Renilla luciferase …

Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely
employed in molecular biology as a reporter gene in cell culture experiments and small …