Precise gene‐editing using CRISPR/Cas9 technology remains a long‐standing challenge,
especially for genes with low expression and no selectable phenotypes in Chlamydomonas …

Chlamydomonas reinhardtii is being transformed from a model organism to an industrial
organism for the production of pigments, fatty acids, and pharmaceuticals. Genetic …

CRISPR-Cas genome engineering in the unicellular green algal model Chlamydomonas
reinhardtii has until now been primarily applied to targeted gene disruption, whereas …

Programmable site-specific nucleases, such as the clustered regularly interspaced short
palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) ribonucleoproteins …

The clustered regularly interspaced short palindromic repeats (CRISPR)–Cas associated
protein 9 (Cas9) system is very precise, efficient and relatively simple in creating genetic …

In recent years, Setaria viridis has been developed as a model plant to better understand the
C4 photosynthetic pathway in major crops. With the increasing availability of genomic …

Genome editing technologies have provided opportunities to manipulate literally any
genomic location, opening new avenues for reverse genetics-based improvements. Among …

CRISPR/Cas9 gene editing in the model green alga Chlamydomonas reinhardtii relies on
the use of selective marker genes to enrich for non-selectable target mutations. This …

Due to their high efficiency, specificity, and flexibility, programmable nucleases, such as
those of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a …

Genome editing is crucial for genetic engineering of organisms for improved traits,
particularly in microalgae due to the urgent necessity for the next generation biofuel …