Biosensor development continues to be driven by the growing need to accurately detect and
monitor analytes with many biotechnology, clinical, agriculture, and military applications …

The research described in this thesis aims at exploiting recent important theoretical and
experimental developments in protein folding towards developing advanced biosensors with …

Protein-based fluorescent biosensors are powerful tools in analyte recognition in vitro and in
vivo. Over the past several decades, advances have been made to produce simplified and …

Proteins that switch conformations in response to a signaling event (eg, ligand binding or
chemical modification) present a unique solution to the design of reagent‐free biosensors as …

Biosensors can be used in applications ranging from identifying disease biomarkers to
detecting spatial and temporal distributions of specific molecules in living cells. A major …

Engineered protein switches have been widely applied in cell-based protein sensors and
point-of-care diagnosis for the rapid and simple analysis of a wide variety of proteins …

Alternate frame folding (AFF) and protein/fragment exchange (FREX) are related
technologies for engineering allosteric conformational changes into proteins that have no …

Protein conformational switches are widely used in functional regulation and biosensing to
visualize biological substances in vitro and in vivo. A major challenge in protein switch …

Engineered protein switches have a large dynamic range, high specificity for the activating
ligand, and a modular architecture, and have been explored for a wide range of applications …

Linkers play critical roles in the construction of synthetic protein switches and sensors as
they functionally couple a receptor with an actuator. With an increasing number of molecular …