In a chamber-based digital PCR (dPCR) chip fabricated with polydimethylsiloxane (PDMS),
bubble generation in the chambers at high temperatures is a critical issue. Here, we found …

We revealed the reason for bubble formation in a PDMS-based digital PCR (dPCR) chip: the
porous PDMS material drives water vapor out of the chip at high temperature and sucks air …

We report a novel Polydimethylsiloxane (PDMS) based digital PCR (dPCR) chip with an
integrated dual-function cavity that accelerates sample loading before the reaction and …

The digital polymerase chain reaction (dPCR) is a high efficacy detection method that is
widely applied in lab-on-a-chip systems. Reported dPCR microfluidic devices usually …

A polydimethylsiloxane (PDMS)-based self-priming microfluidic chip with cushion chambers
is presented in this study for robust and easy-operation digital polymerase chain reaction …

The microfluidic device (MFD) with a glass–PDMS–glass (GPG) structure is of interest for a
wide range of applications. However, GPG MFD fabrication with an ultra-thin PDMS film …

In this paper we demonstrate a new method for microfabricating PDMS devices that controls
vapour diffusion, thereby reducing water loss at elevated temperatures and greatly …

We present a self-discretization and zero-water-loss microfluidic digital PCR (dPCR) device
to enable low-cost and robust quantitative nucleic acid assays. In this device, a thin void is …

This paper reports on the development of a low-cost microreactor (10 µl) biochip for DNA
polymerase chain reaction (PCR). The microbiochip (20 mm× 28 mm) is a hybrid type that is …

A major challenge for the lab-on-a-chip (LOC) community is to develop point-of-care
diagnostic chips that do not use instruments. Such instruments include pumping or liquid …