Molecular diagnosis of helicobacters by PCR is simpler, more accurate, and feasible
compared to other diagnostic methods. Validity and accuracy are highly dependent on the …

The present study aimed to establish a genus-specific PCR-based assay to detect
helicobacters using 16S rRNA gene as the target template. We designed the hemi-nested …

Phenotypic identification of non-pylori Helicobacter species has always been problematic
and time-consuming in comparison with many other bacteria. We developed a rapid two …

Restriction fragment length polymorphism (RFLP) analysis of a∼ 2.6-kbp PCR-amplified
DNA fragment of the 23S (large subunit) ribosomal RNA gene was performed on 92 strains …

Helicobacter is an economically important genus within the phylum Proteobacteria and
include many species which cause many diseases in humans. With the conventional …

Background 16S rRNA amplicon sequencing is an accurate method of detecting microbial
infection without culture. It is unclear if sequencing has additional benefits over routine …

In order to improve simultaneous detection and identification of Helicobacter genus in
general and Helicobacter pylori specifically and reduce the number of amplifications …

Helicobacter pylori is one of the major stomach microbiome components, promoting
development of inflammation and gastric cancer in humans. H. pylori has a unique ability to …

Background. Helicobacter pylori (H. pylori) is ubiquitous among humans and one of the best-
studied examples of an intimate association between bacteria and humans. Phylogeny and …

The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter
genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but …