[HTML][HTML] Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence
T cell exhaustion limits antitumor immunity, but the molecular determinants of this process
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
[HTML][HTML] Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Clinical response to adoptive T cell therapies is associated with the transcriptional and
epigenetic state of the cell product. Thus, discovery of regulators of T cell gene networks and …
epigenetic state of the cell product. Thus, discovery of regulators of T cell gene networks and …
[HTML][HTML] Genome-wide CRISPR screens in primary human T cells reveal key regulators of immune function
Human T cells are central effectors of immunity and cancer immunotherapy. CRISPR-based
functional studies in T cells could prioritize novel targets for drug development and improve …
functional studies in T cells could prioritize novel targets for drug development and improve …
In vitro modeling of CD8+ T cell exhaustion enables CRISPR screening to reveal a role for BHLHE40
Identifying molecular mechanisms of exhausted CD8 T cells (Tex) is a key goal of improving
immunotherapy of cancer and other diseases. However, high-throughput interrogation of in …
immunotherapy of cancer and other diseases. However, high-throughput interrogation of in …
TCR signal strength defines distinct mechanisms of T cell dysfunction and cancer evasion
M Shakiba, P Zumbo, G Espinosa-Carrasco… - Journal of Experimental …, 2021 - rupress.org
T cell receptor (TCR) signal strength is a key determinant of T cell responses. We developed
a cancer mouse model in which tumor-specific CD8 T cells (TST cells) encounter tumor …
a cancer mouse model in which tumor-specific CD8 T cells (TST cells) encounter tumor …
Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion
Understanding complex tissues requires single-cell deconstruction of gene regulation with
precision and scale. Here, we assess the performance of a massively parallel droplet-based …
precision and scale. Here, we assess the performance of a massively parallel droplet-based …
Dynamic chromatin regulatory landscape of human CAR T cell exhaustion
DG Gennert, RC Lynn, JM Granja… - Proceedings of the …, 2021 - National Acad Sciences
Dysfunction in T cells limits the efficacy of cancer immunotherapy. We profiled the
epigenome, transcriptome, and enhancer connectome of exhaustion-prone GD2-targeting …
epigenome, transcriptome, and enhancer connectome of exhaustion-prone GD2-targeting …
[HTML][HTML] In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer
Improving effector activity of antigen-specific T cells is a major goal in cancer
immunotherapy. Despite the identification of several effector T cell (T EFF)-driving …
immunotherapy. Despite the identification of several effector T cell (T EFF)-driving …
T cell exhaustion
A Baessler, DAA Vignali - Annual Review of Immunology, 2024 - annualreviews.org
T cell responses must be balanced to ensure adequate protection against malignant
transformation and an array of pathogens while also limiting damage to healthy cells and …
transformation and an array of pathogens while also limiting damage to healthy cells and …
[HTML][HTML] Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer
CD8+ cytotoxic T cells (CTLs) orchestrate antitumour immunity and exhibit inherent
heterogeneity,, with precursor exhausted T (Tpex) cells but not terminally exhausted T (Tex) …
heterogeneity,, with precursor exhausted T (Tpex) cells but not terminally exhausted T (Tex) …