Introduction. Rapid, reliable and accurate molecular typing methods are essential for
outbreaks detection and infectious diseases control, for monitoring the evolution and …

Background. Routine medical microbiology diagnostics relies on conventional cultivation
followed by phenotypic techniques for identification of pathogenic bacteria and fungi. This is …

Molecular based differentiation of various bacterial species is important in phylogenetic
studies, diagnostics and epidemiological surveillance, particularly where unusual …

Nosocomial infections are a major public health concern worldwide. Early and accurate
identification of nosocomial pathogens which are often multidrug resistant is crucial for …

High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate
DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we …

There is still an ongoing demand for a simple broad-spectrum molecular diagnostic assay
for pathogenic bacteria. For this purpose, we developed a single-plex High Resolution Melt …

The identification of the bacterial species responsible for an infection remains an important
step for the selection of antimicrobial therapy. Gram-negative bacteria are an important …

Background: Broad-range PCR provides valuable information for detecting bacterial
infections. This study assesses the combined use of broad-range real-time PCR and high …

Effective diagnosis and treatment of bloodstream infections are often hampered by a lack of
time-critical information from blood cultures. Molecular techniques aimed at the detection of …

ABSTRACT A real-time PCR assay with the ability to rapidly identify all pathogenic bacteria
would have widespread medical utility. Current real-time PCR technologies cannot …