In vivo cross‐linking of EpsG to EpsL suggests a role for EpsL as an ATPase‐pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae

MD Gray, M Bagdasarian, WGJ Hol… - Molecular …, 2011 - Wiley Online Library
The type II secretion system is a multi‐protein complex that spans the cell envelope of Gram‐
negative bacteria and promotes the secretion of proteins, including several virulence factors …

Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae

ME Yanez, KV Korotkov, J Abendroth… - Journal of molecular …, 2008 - Elsevier
Many Gram-negative bacteria use the multi-protein type II secretion system (T2SS) to
selectively translocate virulence factors from the periplasmic space into the extracellular …

Zinc coordination is essential for the function and activity of the type II secretion ATPase EpsE

CS Rule, M Patrick, JL Camberg, N Maricic… - …, 2016 - Wiley Online Library
The type II secretion system Eps in Vibrio cholerae promotes the extracellular transport of
cholera toxin and several hydrolytic enzymes and is a major virulence system in many Gram …

The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus

ME Yanez, KV Korotkov, J Abendroth… - Journal of molecular …, 2008 - Elsevier
Type II secretion systems (T2SS) translocate virulence factors from the periplasmic space of
many pathogenic bacteria into the extracellular environment. The T2SS of Vibrio cholerae …

Mapping Critical Interactive Sites within the Periplasmic Domain of the Vibrio cholerae Type II Secretion Protein EpsM

TL Johnson, ME Scott, M Sandkvist - Journal of bacteriology, 2007 - Am Soc Microbiol
The type II secretion (T2S) system is present in many gram-negative species, both
pathogenic and nonpathogenic, where it supports the delivery of a variety of toxins …

Secretion of TcpF by the Vibrio cholerae toxin-coregulated pilus biogenesis apparatus requires an N-terminal determinant

CJ Megli, RK Taylor - Journal of bacteriology, 2013 - Am Soc Microbiol
Type IV pili are important for microcolony formation, biofilm formation, twitching motility, and
attachment. We and others have shown that type IV pili are important for protein secretion …

Direct Interaction of the EpsL and EpsM Proteins of the General Secretion Apparatus in Vibrio cholerae

M Sandkvist, LP Hough, MM Bagdasarian… - Journal of …, 1999 - Am Soc Microbiol
The general secretion pathway of gram-negative bacteria is responsible for extracellular
secretion of a number of different proteins, including proteases and toxins. This pathway …

General secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in Vibrio cholerae

M Sandkvist, LO Michel, LP Hough… - Journal of …, 1997 - Am Soc Microbiol
The general secretion pathway (GSP) of Vibrio cholerae is required for secretion of proteins
including chitinase, enterotoxin, and protease through the outer membrane. In this study, we …

Molecular Analysis of the Vibrio cholerae Type II Secretion ATPase EpsE

JL Camberg, M Sandkvist - Journal of bacteriology, 2005 - Am Soc Microbiol
The type II secretion system is a macromolecular assembly that facilitates the extracellular
translocation of folded proteins in gram-negative bacteria. EpsE, a member of this secretion …

Two Regions of EpsL Involved in Species-Specific Protein-Protein Interactions with EpsE and EpsM of the General Secretion Pathway inVibrio cholerae

M Sandkvist, JM Keith, M Bagdasarian… - Journal of …, 2000 - Am Soc Microbiol
Extracellular secretion of proteins via the type II or general secretion pathway in gram-
negative bacteria requires the assistance of at least 12 gene products that are thought to …