High-throughput optical assays for sensing serine hydrolases in living systems and their applications

Q Jin, LL Song, LL Ding, J Zhang, DD Wang… - TrAC Trends in …, 2022 - Elsevier
Serine hydrolases (SHs) comprise a large family of hydrolytic enzymes that play crucial roles
in numerous physiological and pathological processes, such as blood coagulation, cancer …

Serine Hydrolase Activity‐Based Probes for Use in Chemical Proteomics

B Racioppo, N Qiu, A Adibekian - Israel Journal of Chemistry, 2023 - Wiley Online Library
Serine hydrolases (SHs) comprise a large superfamily of enzymes that play critical roles in
many biological processes. Despite their importance, many SHs remain uncharacterized …

Strategies for tuning the selectivity of chemical probes that target serine hydrolases

F Faucher, JM Bennett, M Bogyo, S Lovell - Cell chemical biology, 2020 - cell.com
Serine hydrolases comprise a large family of enzymes that have diverse roles in key cellular
processes, such as lipid metabolism, cell signaling, and regulation of post-translation …

Most recent advances on enzyme‐activatable optical probes for bioimaging

J Mei, H Tian - Aggregate, 2021 - Wiley Online Library
Enzymes are essential biological elements that play vital roles in many key cellular events
and physiological processes. The dysregulation of enzyme activity broadly occurs in a large …

Discovery and evaluation of new activity‐based probes for serine hydrolases

C Wang, D Abegg, BG Dwyer, A Adibekian - ChemBioChem, 2019 - Wiley Online Library
Serine hydrolases play crucial biological roles and are important therapeutic targets in many
clinical applications. Activity‐based protein profiling of serine hydrolases by using …

Chemically diverse activity-based probes with unexpected inhibitory mechanisms targeting trypsin-like serine proteases

A Ramos-Llorca, L Decraecker, VMY Cacheux… - Frontiers in …, 2023 - frontiersin.org
Activity-based probes (ABP) are molecules that bind covalently to the active form of an
enzyme family, making them an attractive tool for target and biomarker identification and …

Comparative assessment of substrates and activity based probes as tools for non-invasive optical imaging of cysteine protease activity

G Blum, RM Weimer, LE Edgington, W Adams… - PLoS …, 2009 - journals.plos.org
Recent advances in the field of non-invasive optical imaging have included the development
of contrast agents that report on the activity of enzymatic targets associated with disease …

Sensitive sulfide ion detection by optofluidic catalytic laser using horseradish peroxidase (HRP) enzyme

C Gong, Y Gong, MKK Oo, Y Wu, Y Rao, X Tan… - Biosensors and …, 2017 - Elsevier
We report an optofluidic catalytic laser for sensitive sulfide ion detection. In the catalytic
reaction, horseradish peroxidase (HRP) enzyme is used for catalyzing the non-fluorescent …

Direct visualization of serine hydrolase activities in complex proteomes using fluorescent active site‐directed probes

MP Patricelli, DK Giang, LM Stamp… - PROTEOMICS …, 2001 - Wiley Online Library
The field of biochemistry is currently faced with the enormous challenge of assigning
functional significance to more than thirty thousand predicted protein products encoded by …

[HTML][HTML] Optical substrates for drug-metabolizing enzymes: Recent advances and future perspectives

Q Jin, JJ Wu, Y Wu, H Li, M Finel, D Wang… - Acta pharmaceutica sinica …, 2022 - Elsevier
Abstract Drug-metabolizing enzymes (DMEs), a diverse group of enzymes responsible for
the metabolic elimination of drugs and other xenobiotics, have been recognized as the …