Image-based profiling is a maturing strategy by which the rich information present in
biological images is reduced to a multidimensional profile, a collection of extracted image …

The increase in imaging throughput, new analytical frameworks and high-performance
computational resources open new avenues for data-rich phenotypic profiling of small …

Highlights•Complex phenotypic imaging assays aim at mimicking the biology of
interest.•Cellular profiling in high-content assays improve description of compound …

Modern drug discovery approaches often use high-content imaging to systematically study
the effect on cells of large libraries of chemical compounds. By automatically screening …

Highlights•Image-based profiling uses hundreds of measurements of cell morphology from
images.•Samples with similar phenotypes are grouped based on this unbiased, quantitative …

Image-based screening is a powerful technique to reveal how chemical, genetic, and
environmental perturbations affect cellular state. Its potential is restricted by the current …

Quantitative analytical approaches for discovering new compound mechanisms are required
for summarizing high-throughput, image-based drug screening data. Here we present a …

Multi-parameter phenotypic profiling of small molecules provides important insights into their
mechanisms of action, as well as a systems level understanding of biological pathways and …

Current drug discovery is dominated by label-dependent molecular approaches, which
screen drugs in the context of a predefined and target-based hypothesis in vitro. Given that …

Profiling approaches such as gene expression or proteome profiling generate small-
molecule bioactivity profiles that describe a perturbed cellular state in a rather unbiased …