Human in vitro oogenesis provides a framework for clarifying the mechanism of human
oogenesis. To create its benchmark, it is vital to promote in vitro oogenesis using a model …

In vitro oogenesis is key to elucidating the mechanism of human female germ‐cell
development and its anomalies. Accordingly, pluripotent stem cells have been induced into …

During fetal oocyte development in mammals, germ cells progress through meiotic prophase
I to form primordial follicles with pregranulosa cells. The primordial follicles remain dormant …

Inactivation of one X chromosome in female mammals (XX) compensates for the reduced
dosage of X-linked gene expression in males (XY). However, the inner cell mass (ICM) of …

Ten to 15% of couples are infertile, with the most common causes being linked to the
production of few or no oocytes or sperm. Yet, our understanding of human germ cell …

The female germ line undergoes a unique sequence of differentiation processes that confers
totipotency to the egg,. The reconstitution of these events in vitro using pluripotent stem cells …

Continuation of mammalian species requires the formation and development of the sexually
dimorphic germ cells. Cultured embryonic stem cells are generally considered pluripotent …

Recent studies have reported the differentiation of pluripotent cells into oocytes in vitro.
However, the developmental competence of in vitro‐generated oocytes remains low. Here …

This protocol is an extension to: Nat. Protoc. 8, 1513–1524 (2013); doi: 10.1038/nprot.
2013.090; published online 11 July 2013 Generation of functional oocytes in culture from …

Reconstituting gametogenesis in vitro is a key goal for reproductive biology and
regenerative medicine. Successful in vitro reconstitution of primordial germ cells and …