Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries

K Lindroos, U Liljedahl, M Raitio… - Nucleic Acids …, 2001 - academic.oup.com
In the microarray format of the minisequencing method multiple oligonucleotide primers
immobilised on a glass surface are extended with fluorescent ddNTPs using a DNA …

Genotyping SNPs by minisequencing primer extension using oligonucleotide microarrays

K Lindroos, U Liljedahl, AC Syvänen - Single Nucleotide Polymorphisms …, 2003 - Springer
A promising approach towards high-throughput genotyping of single nucleotide
polymorphisms (SNPs) is to use arrays of immobilized oligonucleotides in miniaturized …

UV cross-linking of unmodified DNA on glass surfaces

T Schüler, A Nykytenko, A Csaki, R Möller… - Analytical and …, 2009 - Springer
The performance of DNA microarrays strongly depends on their surface properties.
Furthermore, the immobilization method of the capture molecules is of importance for the …

Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer

K Kinoshita, K Fujimoto, T Yakabe, S Saito… - Nucleic acids …, 2007 - academic.oup.com
DNA microarrays are routinely used to monitor gene expression profiling and single
nucleotide polymorphisms (SNPs). However, for practically useful high performance, the …

Attachment of oligonucleotide probes to poly carbodiimide‐coated glass for microarray applications

N Kimura, R Oda, Y Inaki, O Suzuki - Nucleic acids research, 2004 - academic.oup.com
Oligonucleotide‐based DNA microarrays are becoming increasingly useful tools for the
analysis of gene expression and single nucleotide polymorphisms (SNPs). Here, we present …

An inexpensive and simple method for thermally stable immobilization of DNA on an unmodified glass surface: UV linking of poly (T) 10-poly (C) 10–tagged DNA …

H Gudnason, M Dufva, D Duong Bang, A Wolff - Biotechniques, 2008 - Future Science
Microarrays printed on glass slides are often constructed by covalently linking modified
oligonucleotide probes to a derivatized surface at considerable expense. In this article, we …

High reproducibility using sodium hydroxide-stripped long oligonucleotide DNA microarrays

Z Hu, M Troester, CM Perou - Biotechniques, 2005 - Future Science
Recently, long oligonucleotide (60-to 70-mer) microarrays for two-color experiments have
been developed and are gaining widespread use. In addition, when there is limited …

Position of the fluorescent label is a crucial factor determining signal intensity in microarray hybridizations

L Zhang, T Hurek, B Reinhold-Hurek - Nucleic Acids Research, 2005 - academic.oup.com
A key issue in applications of short oligonucleotide-based microarrays is how to design
specific probes with high sensitivity. Some details of the factors affecting microarray …

Fabrication of DNA microarrays using unmodified oligonucleotide probes

DR Call, DP Chandler, F Brockman - BioTechniques, 2001 - Future Science
Microarrays printed on glass slides are often constructed by covalently linking
oligonucleotide probes to a derivatized surface. These procedures typically require relatively …

Factors influencing cDNA microarray hybridization on silylated glass slides

NLW Franssen-van Hal, O Vorst, E Kramer, RD Hall… - Analytical …, 2002 - Elsevier
cDNA microarray technology is becoming the technique of choice for studying gene
expression and gene expression patterns. Although experimental protocols are available …