BACKGROUND: Real-time (RT) PCR methods for discovering and genotyping single
nucleotide polymorphisms (SNPs) are becoming increasingly important in various fields of …

Single-nucleotide polymorphisms (SNPs) and single-nucleotide mutations result from the
substitution of only a single base. The SNP or mutation can be relevant to disease …

Accurate analysis of DNA sequence variation in not only humans and animals but also other
organisms has played a significant role in expanding our knowledge about genetic variety …

Automated, direct cycle sequencing of purified double-stranded PCR products using Taq
polymerase and fluorescently labeled dideoxynucleotide terminators provides a robust and …

The polymerase chain reaction (PCR) is used universally for accurate exponential
amplification of DNA. We describe a high error rate at mononucleotide and dinucleotide …

Allele-specific PCR is considered to be the most straightforward approach for the detection
of SNPs (8, 9). However, its application remains rather limited, mainly because of insufficient …

Tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-
PCR) is a new efficient method for single-nucleotide polymorphism (SNP) genotyping. To …

The quantification of single nucleotide polymorphism (SNP) allele frequencies in pooled
DNA samples using real time PCR is a promising approach for large‐scale diagnostics and …

The specificity and reliability of locked nucleic acid (LNA) substitution at the 3′ position of
allele‐specific PCR (AS‐PCR) primers for SNP detection was investigated in direct …

The increasing need for large-scale genotyping applications of single nucleotide
polymorphisms (SNPs) in model and nonmodel organisms requires the development of low …