Nucleic acid amplification is a powerful molecular biology tool, although its use outside the
modern laboratory environment is limited due to the relatively cumbersome methods …

The complexity of current nucleic acid isolation methods limits their use outside of the
modern laboratory environment. Here, we describe a fast and affordable method to purify …

Global health and food security constantly face the challenge of emerging human and plant
diseases caused by bacteria, viruses, fungi, and other pathogens. Disease outbreaks such …

Modern genotyping techniques, such as SNP analysis and genotyping by sequencing
(GBS), are hampered by poor DNA quality and purity, particularly in challenging plant …

Background Woody tropical plants contain high levels of complex organic compounds that
inhibit the chemical procedures needed to extract RNA or DNA, thus compromising …

Molecular diagnostic tools in the field of food and water quality analysis are becoming
increasingly widespread. Usually, based on DNA amplification techniques such as …

This review highlights various methods that can be used for a sensitive detection of nucleic
acids without using thermal cycling procedures, as is done in PCR or LCR. Topics included …

The isolation of nucleic acids from a biological sample is an important step for many
molecular biology applications and medical diagnostic assays. This chapter describes an …

The extraction of high-quality genomic DNA for PCR amplification from sunflower
(Helianthus annuus) and cotton (Gossypium spp.) is challenging because of the presence of …

Many plant species are considered difficult for DNA isolation due to their high concentrations
of secondary metabolites such as polysaccharides and polyphenols. Several protocols have …