Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations

R Brogna, J Fan, H Sieme, WF Wolkers, H Oldenhof - Scientific reports, 2021 - nature.com
Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin
remains intact. In this study, we investigated drying-and temperature-induced conformational …

Freezing-induced uptake of disaccharides for preservation of chromatin in freeze-dried stallion sperm during accelerated aging

H Oldenhof, M Zhang, K Narten, J Bigalk… - Biology of …, 2017 - academic.oup.com
Nonviable freeze-dried sperm have intact chromatin and can be used for fertilization via
intracytoplasmic sperm injection. Freeze-dried sperm preferably should be stored at 4° C or …

Trehalose attenuates detrimental effects of freeze-drying on human sperm parameters

E Shahmoradi, N Baheiraei, I Halvaei - Biopreservation and …, 2022 - liebertpub.com
Freeze-drying is one of the sperm preservation methods leading to the long-term
preservation of sperm genetic material. Our main goal of this study was to evaluate the effect …

Ultrastructural modification of ram sperm frozen with cyclohexanediol and trehalose

J Liang, C Lv, H Yang, Y Zhang, SHA Raza… - Biopreservation and …, 2022 - liebertpub.com
In this study, the effects of trehalose and 1, 3-cyclohexanediol (1, 3-CHD) on the
ultrastructure of frozen-thawed ram sperm were assessed and compared. In the control …

Beneficial effects of trehalose and gentiobiose on human sperm cryopreservation

D Gholami, M Sharafi, V Esmaeili, T Nadri, L Alaei… - Plos one, 2023 - journals.plos.org
The protection of human sperm during cryopreservation is of great importance to infertility.
Recent studies have shown that this area is still a long way from its ultimate aim of …

[HTML][HTML] Effects of cryopreservation on human sperm deoxyribonucleic acid integrity

N Zribi, NF Chakroun, H El Euch, J Gargouri… - Fertility and sterility, 2010 - Elsevier
OBJECTIVE: To evaluate the effect of cryopreservation on sperm motility and viability and to
assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation …

The effect of two cryopreservation methods on human sperm DNA damage

T Liu, J Gao, N Zhou, M Mo, X Wang, X Zhang, H Yang… - Cryobiology, 2016 - Elsevier
Several methods are currently available for selection when conducting sperm
cryopreservation, however, these methods might cause different degrees of damage on …

Effect of different disaccharides on the integrity and fertilising ability of freeze-dried boar spermatozoa: a preliminary study

A García, L Gil, C Malo, F Martínez… - …, 2014 - ingentaconnect.com
Freeze-drying spermatozoa is a developing technique that facilitates semen storage and
transport. However, freeze-dried sperm exhibits impaired DNA integrity, which is associated …

Effect of trehalose on DNA integrity of freeze-dried boar sperm, fertilization, and embryo development after intracytoplasmic sperm injection

NT Men, K Kikuchi, M Nakai, A Fukuda, F Tanihara… - Theriogenology, 2013 - Elsevier
Freeze-drying (FD) medium containing ethylene glycol-bis (2-aminoethylether)-N, N, N′,
N′-tetraacetic acid (EGTA) is reported to be beneficial for maintenance of sperm DNA …

Freeze-dried dog sperm: Dynamics of DNA integrity

M Olaciregui, V Luño, N Gonzalez, I De Blas, L Gil - Cryobiology, 2015 - Elsevier
Freeze-drying (FD) has been proposed as an alternative method to preserve spermatozoa.
During the FD procedure, sperm DNA might become damaged by both freezing and drying …