Measuring Novel Protein-Protein Binding with Surface Plasmon Resonance in the Physical Chemistry Lab

BJ McFarland - Engaging Students in Physical Chemistry, 2018 - ACS Publications
In the laboratory for Survey of Physical Chemistry, students proceeded through a five-week
project in which they measured protein-protein binding. This project engaged the students in …

A CURE biochemistry laboratory module to study protein–protein interactions by NMR spectroscopy

WM Marsiglia, R Qamra, KM Jackson… - Journal of chemical …, 2020 - ACS Publications
The design of undergraduate laboratory courses that provide meaningful research-based
experiences enhances undergraduate curricula and prepares future graduate students for …

[HTML][HTML] The impact of different human IgG capture molecules on the kinetics analysis of antibody-antigen interaction

V Kamat, A Rafique, T Huang, O Olsen, W Olson - Analytical biochemistry, 2020 - Elsevier
Surface plasmon resonance (SPR) is a well-established method to characterize
biomolecular interactions and is widely used in drug discovery and development. Here, we …

Real time measurements of membrane protein: receptor interactions using surface plasmon resonance (SPR)

NL Levanon, E Vigonsky, O Lewinson - JoVE (Journal of Visualized …, 2014 - jove.com
Protein-protein interactions are pivotal to most, if not all, physiological processes, and
understanding the nature of such interactions is a central step in biological research …

Measuring antibody-antigen binding kinetics using surface plasmon resonance

S Hearty, P Leonard, H Ma, R O'Kennedy - … Engineering: Methods and …, 2018 - Springer
Surface plasmon resonance (SPR) is now widely embraced as a technology for monitoring a
diverse range of protein-protein interactions and is considered almost de rigueur for …

[HTML][HTML] ABRF–MIRG Benchmark Study: Molecular Interactions in a Three-Component System

AP Yamniuk, SC Edavettal, S Bergqvist… - Journal of …, 2012 - ncbi.nlm.nih.gov
Protein–protein interactions identified through high-throughput proteomics efforts continue to
advance our understanding of the protein interactome. In addition to highly specific protein …

Measuring antibody–antigen binding kinetics using surface plasmon resonance

S Hearty, P Leonard, R O'Kennedy - Antibody Engineering: Methods and …, 2012 - Springer
Surface plasmon resonance (SPR) is now widely embraced as a technology for monitoring a
diverse range of protein–protein interactions and is considered almost de rigueur for …

Biophysical characterization of protein-protein interactions with mass photometry

F Soltermann - 2020 - ora.ox.ac.uk
Interactions between biomolecules control the processes of life in health and their
malfunction in disease, making their characterization and quantification essential. We first …

Kinetics and binding geometries of the complex between β2-microglobulin and its antibody: An AFM and SPR study

E Coppari, S Santini, AR Bizzarri, S Cannistraro - Biophysical Chemistry, 2016 - Elsevier
Abstract β 2-Microglobulin (B2M) is a human protein involved in the regulation of immune
response and represents a useful biomarker for several diseases. Recently, anti-B2M …

An overview of current methods to confirm protein-protein interactions

K Miura - Protein and peptide letters, 2018 - ingentaconnect.com
Background: The research field of protein-protein interactions is interdisciplinary and
specialized field that spans all aspects of biology, physics and chemistry. Therefore, in order …