Reproducing the environment to which the embryo is naturally exposed may be an
alternative to improve viability of embryos produced in vitro. In the first part of this work, we …

Profiling bovine blastocyst transcriptome at the single-cell level has enabled us to reveal the
first cell lineage segregation, during which the inner cell mass (ICM), trophectoderm (TE) …

It is well known that serum in culture medium negatively affects blastocyst quality. The
objective of this work was to develop and test a serum-free culture medium which could …

Two major considerations governing choice of culture system for in vitro production of
bovine and other mammalian embryos are the efficiency of blastocyst production and the …

In vitro production of livestock embryos is important for both agriculture and biomedical
research. In spite advances in designing media and culture systems, we do not yet know …

Over the past decades in vitro production (IVP) of bovine embryos has been significantly
improved. Nevertheless, embryos generated in vitro still differ from their in vivo produced …

Bovine in vitro embryo production (IVP) is an animal biotechnology important for both
agriculture and biomedical research. IVP comprises ultrasound-guided oocyte recovery from …

Recent studies in our laboratory have indicated that bovine embryos only use a small
amount of the nutrients available to them in culture. Our objective was to evaluate the …

In experiment 1, the effects of a group of either 20 (ie glutamine+ essential+ non-essential)
or 11 (ie hamster embryo culture medium (HECM)-6) amino acids were evaluated in …

Abstract Development of in vitro-produced bovine embryos was studied in 3 two-step culture
media: synthetic oviduct fluid (SOF), Gardner's G1/G2, and control (hamster embryo culture …