Bacterial species and communities play foundational roles in human health and
therapeutics, in vital ecological and environmental processes, and in industrial applications …

CRISPR-associated transposons (CASTs) have the potential to transform the technology
landscape for kilobase-scale genome engineering, by virtue of their ability to integrate large …

Genome engineering tools in bacteria are limited by their targeting abilities, cargo size
capacities, and integration efficiencies. Programmable Cas-directed transposons have been …

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated
transposases have the potential to transform the technology landscape for kilobase-scale …

Existing technologies for site-specific integration of kilobase-sized DNA sequences in
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …

The transposition mechanism of the Vibrio cholerae Tn6677 transposon (VcTn6677) is
based on the unique synergy between a classical transposition machinery (TnsABC-TniQ …

CRISPR-Cas systems generally provide adaptive immunity in prokaryotes through RNA-
guided degradation of foreign genetic elements like bacteriophages and plasmids. Recently …

Transposons have been instrumental tools in microbiology enabling random mutagenesis,
with transposons like Tn5 and Mariner, and site-specific DNA integrations with Tn7 …

CRISPR-Cas can target a specific sequence of DNA or RNA guided by a short RNA guide.
The target sites can be changed with the RNA sequences. A variety of powerful genetic tools …

Facile bacterial genome sequencing has unlocked a veritable treasure trove of novel genes
awaiting functional exploration. To make the most of this opportunity requires powerful …