Deoxycholic acid (DCA) has been authorized by the Federal Drug Agency for cosmetic
reduction of redundant submental fat. The hydroxylated product (6β-OH DCA) was …

Regio‐and stereo‐selective hydroxylation of bile acids is a valuable reaction but often lacks
suitable catalysts. In the research, semi‐rational design in protein engineering techniques …

We engineered the cytochrome P450 monooxygenase CYP107D1 (OleP) from
Streptomyces antibioticus for the stereo‐and regioselective 7β‐hydroxylation of lithocholic …

Ursodeoxycholic acid (UDCA) is a bile acid of industrial interest as it is used as an agent for
the treatment of primary sclerosing cholangitis and the medicamentous, non‐surgical …

Abstract E. coli (P450pyrTM‐GDH) with dual plasmids, pETDuet containing P450pyr triple
mutant I83H/M305Q/A77S (P450pyrTM) and ferredoxin reductase (FdR) genes and …

Engineering of a hydroxylase for highly active and stereoselective biohydroxylation of C
(sp3)–H bonds attracts keen interest in synthetic chemistry. Herein, we report the …

Abstract 7α-and 7β-hydroxysteroid dehydrogenases (HSDHs) are key biocatalysts for the
biotransformation of ursodeoxycholic acid (UDCA) from chenodeoxycholic acid (CDCA) …

The selective oxyfunctionalization of unactivated CH bonds is one of long-standing issues
and current topics in synthetic chemistry. One of the major synthetic targets for these …

ABSTRACT CYP105D18 supports H2O2 as an oxygen surrogate for catalysis well and
shows high H2O2 resistance capacity. We report the hydroxylation of different steroids using …

Substrate engineered, achiral carboxylic acid derivative 2 was biohydroxylated with various
mutants of cytochrome P450 BM-3 to give two out of the four possible diastereoisomers in …