In vitro mouse spermatogenesis with an organ culture method in chemically defined medium
H Sanjo, M Komeya, T Sato, T Abe, K Katagiri… - PLoS …, 2018 - journals.plos.org
We previously reported the successful induction and completion of mouse spermatogenesis
by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential …
by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential …
Fact or fiction: In vitro spermatogenesis
K Reuter, S Schlatt, J Ehmcke, J Wistuba - Spermatogenesis, 2012 - Taylor & Francis
Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro,
but no efficient system has been established yet that covers the entire process of …
but no efficient system has been established yet that covers the entire process of …
In vitro spermatogenesis: A century‐long research journey, still half way around
M Komeya, T Sato, T Ogawa - Reproductive medicine and …, 2018 - Wiley Online Library
Background Spermatogenesis is one of the most complicated cellular differentiation
processes in a body. Researchers struggled to find and develop a micro‐environmental …
processes in a body. Researchers struggled to find and develop a micro‐environmental …
In vitro murine spermatogenesis in an organ culture system
A Gohbara, K Katagiri, T Sato, Y Kubota… - Biology of …, 2010 - academic.oup.com
Achieving mammalian spermatogenesis in vitro has a long history of research but remains
elusive. The organ culture method has advantages over the cell culture method, because …
elusive. The organ culture method has advantages over the cell culture method, because …
The fertilising ability of spermatogenic cells derived from cultured mouse immature testicular tissue
S Suzuki, K Sato - Zygote, 2003 - cambridge.org
There are many reports about the in vitro culture of spermatogenic cells, but no-one has
succeeded in inducing the differentiation from spermatogonia to intact sperm. Also the study …
succeeded in inducing the differentiation from spermatogonia to intact sperm. Also the study …
In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues
M Komeya, H Yamanaka, H Sanjo… - Reproductive …, 2019 - Wiley Online Library
Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods,
by placing the testis tissue at the interphase between culture medium and air. In this …
by placing the testis tissue at the interphase between culture medium and air. In this …
In vitro spermatogenesis using an organ culture technique
T Yokonishi, T Sato, K Katagiri, T Ogawa - Spermatogenesis: Methods and …, 2013 - Springer
Research on in vitro spermatogenesis has a long history and remained to be an
unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm …
unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm …
[HTML][HTML] Differentiation of murine male germ cells to spermatozoa in a soft agar culture system
MA Elhija, E Lunenfeld, S Schlatt… - Asian journal of …, 2012 - ncbi.nlm.nih.gov
Establishment of an in vitro system that allows the development of testicular germ cells to
sperm will be valuable for studies of spermatogenesis and future treatments for male …
sperm will be valuable for studies of spermatogenesis and future treatments for male …
Organ culture of seminiferous tubules using a modified soft agar culture system
K Gholami, G Pourmand, M Koruji, S Ashouri… - Stem cell research & …, 2018 - Springer
Background In-vitro spermatogenesis in mammalian species is considered an important
topic in reproductive biology. New strategies for achieving a complete version of …
topic in reproductive biology. New strategies for achieving a complete version of …
Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
T Matsumura, T Sato, T Abe, H Sanjo, K Katagiri… - Scientific Reports, 2021 - nature.com
In vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible
with mouse testis tissues. The same method, however, has been hardly applicable to …
with mouse testis tissues. The same method, however, has been hardly applicable to …