We previously reported the successful induction and completion of mouse spermatogenesis
by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential …

Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro,
but no efficient system has been established yet that covers the entire process of …

Background Spermatogenesis is one of the most complicated cellular differentiation
processes in a body. Researchers struggled to find and develop a micro‐environmental …

Achieving mammalian spermatogenesis in vitro has a long history of research but remains
elusive. The organ culture method has advantages over the cell culture method, because …

There are many reports about the in vitro culture of spermatogenic cells, but no-one has
succeeded in inducing the differentiation from spermatogonia to intact sperm. Also the study …

Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods,
by placing the testis tissue at the interphase between culture medium and air. In this …

Research on in vitro spermatogenesis has a long history and remained to be an
unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm …

Establishment of an in vitro system that allows the development of testicular germ cells to
sperm will be valuable for studies of spermatogenesis and future treatments for male …

Background In-vitro spermatogenesis in mammalian species is considered an important
topic in reproductive biology. New strategies for achieving a complete version of …

In vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible
with mouse testis tissues. The same method, however, has been hardly applicable to …