Development of a Multiplex PCR Technique for Detection and Epidemiological Typing of Salmonella in Human Clinical Samples
J Alvarez, M Sota, AB Vivanco, I Perales… - Journal of Clinical …, 2004 - Am Soc Microbiol
We have developed a multiplex PCR assay for Salmonella detection and epidemiological
typing. Six sets of primers were designed to detect the major Salmonella serotypes and …
typing. Six sets of primers were designed to detect the major Salmonella serotypes and …
Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR
SH Park, HJ Kim, WH Cho, JH Kim… - FEMS microbiology …, 2009 - academic.oup.com
This study was designed to develop a multiplex PCR method with five specific primer pairs
for the detection of Salmonella spp., Salmonella subspecies I, Salmonella enterica serovars …
for the detection of Salmonella spp., Salmonella subspecies I, Salmonella enterica serovars …
Multiplex PCR-Based Method for Identification of Common Clinical Serotypes of Salmonella enterica subsp. enterica
S Kim, JG Frye, J Hu, PJ Fedorka-Cray… - Journal of clinical …, 2006 - Am Soc Microbiol
ABSTRACT A multiplex PCR method has been developed to differentiate between the most
common clinical serotypes of Salmonella enterica subsp. enterica encountered in …
common clinical serotypes of Salmonella enterica subsp. enterica encountered in …
Rapid identification of Salmonella enterica serovars, typhimurium, choleraesuis, infantis, hadar, enteritidis, dublin and gallinarum, by multiplex PCR
M Akiba, M Kusumoto, T Iwata - Journal of microbiological methods, 2011 - Elsevier
Salmonella enterica subsp. enterica poses a threat to both human and animal health, with
more than 2500 serovars having been reported to date. Salmonella serovars are identified …
more than 2500 serovars having been reported to date. Salmonella serovars are identified …
Comparison of Five Repetitive-Sequence-Based PCR Typing Methods for Molecular Discrimination of Salmonellaenterica Isolates
G Rasschaert, K Houf, H Imberechts… - Journal of Clinical …, 2005 - Am Soc Microbiol
Five repetitive-element PCR (rep-PCR) techniques [primer sets ERIC1R-ERIC2 and REP1R-
REP2I and primers ERIC2, BOXA1R, and (GTG) 5] were evaluated for the discrimination of …
REP2I and primers ERIC2, BOXA1R, and (GTG) 5] were evaluated for the discrimination of …
Automated 5′ Nuclease PCR Assay for Identification of Salmonella enterica
J Hoorfar, P Ahrens, P Rådstrom - Journal of clinical microbiology, 2000 - Am Soc Microbiol
ABSTRACT A simple and ready-to-go test based on a 5′ nuclease (TaqMan) PCR
technique was developed for identification of presumptive Salmonella enterica isolates. The …
technique was developed for identification of presumptive Salmonella enterica isolates. The …
Detection of Salmonella serovars from clinical samples by enrichment broth cultivation-PCR procedure
GG Stone, RD Oberst, MP Hays, S Mcvey… - Journal of Clinical …, 1994 - Am Soc Microbiol
To overcome problems associated with application of PCR to clinical samples, we have
combined a short cultivation procedure with a Salmonella-specific PCR-hybridization assay …
combined a short cultivation procedure with a Salmonella-specific PCR-hybridization assay …
Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR …
CH Chiu, JT Ou - Journal of clinical microbiology, 1996 - Am Soc Microbiol
In order to make a rapid and definite diagnosis of Salmonella enteritis in children, an
enrichment broth culture-multiplex PCR combination assay was devised to identify …
enrichment broth culture-multiplex PCR combination assay was devised to identify …
High-Throughput Molecular Determination of Salmonella enterica Serovars by Use of Multiplex PCR and Capillary Electrophoresis Analysis
Salmonella enterica is a leading cause of food-borne illness worldwide and is also a major
cause of morbidity and mortality in domestic and wild animals. In the current study, a high …
cause of morbidity and mortality in domestic and wild animals. In the current study, a high …
A rapid and direct real time PCR-based method for identification of Salmonella spp.
D Rodrı́guez-Lázaro, M Hernández, T Esteve… - Journal of …, 2003 - Elsevier
The aim of this work was the validation of a rapid, real-time PCR assay based on TaqMan®
technology for the unequivocal identification of Salmonella spp. to be used directly on an …
technology for the unequivocal identification of Salmonella spp. to be used directly on an …