Detection of Aspergillus fumigatus by polymerase chain reaction

C Spreadbury, D Holden… - Journal of Clinical …, 1993 - Am Soc Microbiol
Aspergillus fumigatus is an opportunistic nosocomial pathogen causing an often fatal
pneumonia, invasive aspergillosis (IA), in immunosuppressed patients. Oligonucleotide …

Development of a LightCycler PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples from Neutropenic Patients

B Spiess, D Buchheidt, C Baust… - Journal of clinical …, 2003 - Am Soc Microbiol
The increasing incidence of invasive aspergillosis, a life-threatening infection in
immunocompromised patients, emphasizes the need to improve the diagnostic tools for this …

Semiquantitative Detection by Real-Time PCR of Aspergillus fumigatus in Bronchoalveolar Lavage Fluids and Tissue Biopsy Specimens from Patients with Invasive …

K Rantakokko-Jalava, S Laaksonen… - Journal of clinical …, 2003 - Am Soc Microbiol
ABSTRACT A real-time PCR method was developed and used to detect Aspergillus
fumigatus mitochondrial DNA (mtDNA) in bronchoalveolar lavage (BAL) fluids and tissue …

Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR

S Bretagne, JM Costa… - Journal of clinical …, 1995 - Am Soc Microbiol
A competitive PCR assay involving the use of bronchoalveolar lavage (BAL) samples for the
diagnosis of invasive pulmonary aspergillosis (IPA) was developed. For this purpose, a 1-kb …

General primer-mediated PCR for detection of Aspergillus species

WJ Melchers, PE Verweij, P Van den Hurk… - Journal of clinical …, 1994 - Am Soc Microbiol
A PCR assay was developed for the diagnosis of invasive aspergillosis in
immunocompromised patients. For this purpose, the complete nucleotide sequences of the …

In-situ detection of Aspergillus fumigatus

R Hanazawa, SY Murayama… - Journal of Medical …, 2000 - microbiologyresearch.org
An in-situ hybridisation (ISH) technique to detect Aspergillus fumigatus in infected tissues
was developed in which 568-bp, 333-bp and 154-bp PCR products of the alkaline …

Identification of Aspergillus fumigatus and Related Species by Nested PCR Targeting Ribosomal DNA Internal Transcribed Spacer Regions

J Zhao, F Kong, R Li, X Wang, Z Wan… - Journal of clinical …, 2001 - Am Soc Microbiol
Aspergillus fumigatus is the most common species that causes invasive aspergillosis. In
order to identify A. fumigatus, partial ribosomal DNA (rDNA) from two to six strains of five …

Specific Detection of Aspergillus Species in Blood and Bronchoalveolar Lavage Samples of Immunocompromised Patients by Two-Step PCR

H Skladny, D Buchheidt, C Baust… - Journal of clinical …, 1999 - Am Soc Microbiol
The increasing incidence of aspergillosis, a life-threatening infection in
immunocompromised patients, emphasizes the need to improve the currently limited …

Detection of Aspergillus fumigatus in a Rat Model of Invasive Pulmonary Aspergillosis by Real-Time Nucleic Acid Sequence-Based Amplification

Y Zhao, S Park, P Warn, R Shrief… - Journal of clinical …, 2010 - Am Soc Microbiol
Rapid and sensitive detection of Aspergillus from clinical samples may facilitate the early
diagnosis of invasive pulmonary aspergillosis (IPA). A real-time nucleic acid sequence …

Detection of Aspergillus Species in Blood and Bronchoalveolar Lavage Samples from Immunocompromised Patients by Means of 2-Step Polymerase Chain Reaction …

D Buchheidt, C Baust, H Skladny, J Ritter… - Clinical infectious …, 2001 - academic.oup.com
Bronchoalveolar lavage (BAL) samples from 67 patients who were at high risk for invasive
aspergillosis were examined using a recently developed 2-step polymerase chain reaction …