A compact Cascade–Cas3 system for targeted genome engineering
CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …
[HTML][HTML] CRISPR-assisted editing of bacterial genomes
The targeting of nucleases to specific DNA sequences facilitates genome editing. Recent
work demonstrated that the CRISPR-associated (Cas) nuclease Cas9 can be targeted to …
work demonstrated that the CRISPR-associated (Cas) nuclease Cas9 can be targeted to …
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations …
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations …
[HTML][HTML] Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Targeted genome editing technologies have enabled a broad range of research and
medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted …
medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted …
[HTML][HTML] Genome editing in bacteria: CRISPR-Cas and beyond
RD Arroyo-Olarte, R Bravo Rodriguez, E Morales-Ríos - Microorganisms, 2021 - mdpi.com
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
Bacterial RNA–directed Cas9 endonuclease is a versatile tool for site-specific genome
modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and …
modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and …
[HTML][HTML] In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present
the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni …
the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni …
[HTML][HTML] Endogenous CRISPR-Cas system-based genome editing and antimicrobials: review and prospects
Y Li, N Peng - Frontiers in microbiology, 2019 - frontiersin.org
CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
Programmed genome editing by a miniature CRISPR-Cas12f nuclease
Z Wu, Y Zhang, H Yu, D Pan, Y Wang, Y Wang… - Nature chemical …, 2021 - nature.com
The RNA-guided CRISPR-associated (Cas) nucleases are versatile tools for genome editing
in various organisms. The large sizes of the commonly used Cas9 and Cas12a nucleases …
in various organisms. The large sizes of the commonly used Cas9 and Cas12a nucleases …
[HTML][HTML] Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …