The phage lambda-derived Red recombination system is a powerful tool for making targeted
genetic changes in Escherichia coli, providing a simple and versatile method for generating …

Abstract Background The Red proteins of lambda phage mediate probably the simplest and
most efficient homologous recombination reactions yet described. However the mechanism …

A phage λ-based recombination system, Red, can be used for high-efficiency mutagenesis,
repair, and engineering of chromosomal or episomal DNA in vivo in Escherichia coli. When …

The bacteriophage λ Red homologous recombination system has been studied over the
past 50 years as a model system to define the mechanistic details of how organisms …

Homologous recombination can be used to generate recombinants on episomes or directly
on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA …

Lambda Red recombineering is a powerful technique for making targeted genetic changes
in bacteria. However, many applications are limited by the frequency of recombination …

Recent studies have generated interest in the use of the homologous recombination system
of bacteriophage λ for genetic engineering. The system, called Red, consists primarily of …

Background Homologous recombination mediated by the λ-Red genes is a common method
for making chromosomal modifications in Escherichia coli. Several protocols have been …

Genetic recombination catalyzed by λ's Red pathway was studied in rec+ and recA mutant
bacteria by examining both intracellular λ DNA and mature progeny particles …

Abstract Background The Red recombinase system of bacteriophage lambda has been
used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists …