Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Ananas comosus var. bracteatus During Chimeric Leaf Development and Response …

M Mao, Y Xue, Y He, X Zhou, H Hu, J Liu, L Feng… - Frontiers in …, 2021 - frontiersin.org
Reverse transcription quantitative real-time PCR (RT-qPCR) is a common way to study gene
regulation at the transcriptional level due to its sensibility and specificity, but it needs …

Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditions

V Mafra, KS Kubo, M Alves-Ferreira, M Ribeiro-Alves… - PloS one, 2012 - journals.plos.org
Real-time reverse transcription PCR (RT-qPCR) has emerged as an accurate and widely
used technique for expression profiling of selected genes. However, obtaining reliable …

Genome-Wide Identification and Evaluation of New Reference Genes in Pineapple (Ananas comosus L.) during Stamen and Ovule Development

X Jin, Z Hou, L Zhao, L Liu, S Priyadarshani… - Tropical Plant …, 2020 - Springer
Quantitative real-time PCR (qRT-PCR) is a convenient tool for gene expression analysis.
However, reliability of its result is influenced largely by the selection of reference genes …

Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time PCR

M Hu, W Hu, Z Xia, X Zhou, W Wang - Frontiers in Plant Science, 2016 - frontiersin.org
Reverse transcription quantitative real-time polymerase chain reaction (real-time PCR, also
referred to as quantitative RT-PCR or RT-qPCR) is a highly sensitive and high-throughput …

Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions

L Chen, H Zhong, J Kuang, J Li, W Lu, J Chen - Planta, 2011 - Springer
Reverse transcription quantitative real-time PCR (RT-qPCR) is a sensitive technique for
quantifying gene expression, but its success depends on the stability of the reference gene …

Identification and evaluation of reference genes for reliable normalization of real-time quantitative PCR data in acerola fruit, leaf, and flower

CP Dos Santos, KD da Cruz Saraiva, MC Batista… - Molecular biology …, 2020 - Springer
Understanding into acerola (Malpighia emarginata) molecular and biochemical bases is still
obscure, despite it is one of the most important natural source of vitamin C for humans …

Validation of Reference Genes Aiming Accurate Normalization of qRT-PCR Data in Dendrocalamus latiflorus Munro

M Liu, J Jiang, X Han, G Qiao, R Zhuo - PLoS One, 2014 - journals.plos.org
Background Dendrocalamus latiflorus Munro distributes widely in subtropical areas and
plays vital roles as valuable natural resources. The transcriptome sequencing for D. latiflorus …

Selection of reference genes for quantitative real-time PCR normalization in Narcissus pseudonarcissus in different cultivars and different organs

X Li, D Tang, Y Shi - Heliyon, 2018 - cell.com
Quantitative real-time PCR (qRT-PCR) has been a widely used accurate technique for gene
expression analysis in various species. However, its results require data normalization by …

Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions

Y Zhang, X Peng, Y Liu, Y Li, Y Luo, X Wang… - BMC Molecular …, 2018 - Springer
Background Strawberry has received much attention due to its nutritional value, unique
flavor, and attractive appearance. The availability of the whole genome sequence and …

Selection of suitable reference genes for reverse transcription quantitative real-time PCR studies on different experimental systems from carrot (Daucus carota L.)

MD Campos, AM Frederico, T Nothnagel… - Scientia …, 2015 - Elsevier
Reverse transcription quantitative real time polymerase chain reaction (RT-qPCR) is a
preferred method for rapid and accurate quantification of gene expression studies …