Unraveling the mechanisms of microbial adaptive evolution following genetic or
environmental challenges is of fundamental interest in biological science and engineering …

ABSTRACT A mechanistic understanding of how new phenotypes develop to overcome the
loss of a gene product provides valuable insight on both the metabolic and regulatory …

The ability of biological systems to adapt to genetic and environmental perturbations is a
fundamental but poorly understood process at the molecular level. By quantifying metabolic …

Comparative whole-genome sequencing enables the identification of specific mutations
during adaptation of bacteria to new environments and allelic replacement can establish …

Bacterial survival requires adaptation to different environmental perturbations such as
exposure to antibiotics, changes in temperature or oxygen levels, DNA damage, and …

Adaptive laboratory evolution (ALE) is a widely-used method for improving the fitness of
microorganisms in selected environmental conditions. It has been applied previously to …

Adaptive laboratory evolution (ALE) has emerged as a new approach with which to pursue
fundamental biological inquiries and, in particular, new insights into the systemic function of …

Highlights•Adaptive laboratory evolution (ALE) is a powerful tool in basic research and strain
development.•ALE studies are most informative when fitness improvement are elucidated by …

Evolutionary adaptation to a constant environment is often accompanied by specialization
and a reduction of fitness in other environments. We assayed the ability of the Lenski …

Transhydrogenases catalyse interconversion of the redox cofactors NADH and NADPH,
thereby conveying metabolic flexibility to balance catabolic NADPH formation with anabolic …